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Cell Signal Transduction Research Antibodies
RFC1 Polyclonal Antibody
Polyclonal, Rabbit
$225/ 100ul
QuantityTotal: $225
Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.
Product Information
Molecular Weight128255Da
Protein NameReplication factor C subunit 1
Human Gene Id5981
Human Swiss Prot NoP35251
Mouse Swiss Prot NoP35601
SpecificityRFC1 Polyclonal Antibody detects endogenous levels of RFC1 protein.
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration1 mg/ml
Storage Stability-20°C/1 year
Other NameRFC1; RFC140; Replication factor C subunit 1; Activator 1 140 kDa subunit; A1 140 kDa subunit; Activator 1 large subunit; Activator 1 subunit 1; DNA-binding protein PO-GAReplication factor C 140 kDa subunit; RF-C 140 kDa subunit; RFC140; Replication factor C large subunit
Observed Band(KD)130
FunctionInteracts with C-terminus of PCNA. 5' phosphate residue is required for binding of the N-terminal DNA-binding domain to duplex DNA, suggesting a role in recognition of non-primer template DNA structures during replication and/or repair.,function:The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins PCNA and activator 1. This subunit binds to the primer-template junction. Binds the PO-B transcription element as well as other GA rich DNA sequences. Could play a role in DNA transcription regulation as well as DNA replication and/or repair. Can bind single- or double-stranded DNA.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the activator 1 large subunit family.,similarity:Contains 1 BRCT domain.,subunit:Heterotetramer of subunits RFC2, RFC3, RFC4 and RFC5 that can form a complex
Subcellular LocationNucleus.
ExpressionWide tissue distribution. Undetectable in placental tissue.
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
ImmunogenThe antiserum was produced against synthesized peptide derived from the C-terminal region of human RFC1. AA range:1071-1120
Gene NameRFC1
DilutionWestern Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.
Backgroundreplication factor C subunit 1(RFC1) Homo sapiens This gene encodes the large subunit of replication factor C, a five subunit DNA polymerase accessory protein, which is a DNA-dependent ATPase required for eukaryotic DNA replication and repair. The large subunit acts as an activator of DNA polymerases, binds to the 3' end of primers, and promotes coordinated synthesis of both strands. It may also have a role in telomere stability. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Mar 2011],
Conjugated Antibody
This product is also available conjugated. Format: E20-75385-Conjugate. Price: $485 / Size: 100μl.
E20-75385-FITC$485 / 100μlE20-75385-Biotin$485 / 100μlE20-75385-HRP$485 / 100μlE20-75385-AP$485 / 100μlE20-75385-AF350$485 / 100μlE20-75385-AF405M$485 / 100μlE20-75385-AF488$485 / 100μlE20-75385-AF514$485 / 100μlE20-75385-AF532$485 / 100μlE20-75385-AF555$485 / 100μlE20-75385-AF568$485 / 100μlE20-75385-AF405S$485 / 100μlE20-75385-AF405L$485 / 100μlE20-75385-AF546$485 / 100μlE20-75385-AF594$485 / 100μlE20-75385-AF610$485 / 100μlE20-75385-AF635$485 / 100μlE20-75385-AF647$485 / 100μlE20-75385-AF680$485 / 100μlE20-75385-AF700$485 / 100μlE20-75385-AF750$485 / 100μlE20-75385-AF790$485 / 100μlE20-75385-APC$485 / 100μlE20-75385-PE$485 / 100μlE20-75385-Cy3$485 / 100μlE20-75385-Cy5$485 / 100μlE20-75385-Cy5.5$485 / 100μlE20-75385-Cy7$485 / 100μlE20-75385-PE-Cy5$485 / 100μl
Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.
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