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Cell Signal Transduction Research Antibodies
NFATc1 Polyclonal Antibody
Polyclonal, Rabbit
$225/ 100ul
QuantityTotal: $225
Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.
Product Information
Molecular Weight101243Da
Protein NameNuclear factor of activated T-cells cytoplasmic 1
Human Gene Id4772
Human Swiss Prot NoO95644
Mouse Swiss Prot NoO88942
SpecificityNFATc1 Polyclonal Antibody detects endogenous levels of NFATc1 protein.
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration1 mg/ml
Storage Stability-20°C/1 year
Other NameNFATC1; NFAT2; NFATC; Nuclear factor of activated T-cells, cytoplasmic 1; NF-ATc1; NFATc1; NFAT transcription complex cytosolic component; NF-ATc; NFATc
Observed Band(KD)105
Functionalternative products:Isoform C-alpha and isoform C-beta are the strongest activator of gene transcription, followed by isoform A-alpha and isoform A-beta, whereas isoform B-alpha and isoform B-beta are the weakest. Isoform B-alpha, isoform B-beta, isoform C-alpha and isoform C-beta, both present in T-cells, can modulate their transcriptional activity,domain:Isoforms C have a C-terminal part with an additional trans-activation domain, TAD-B, which acts as a transcriptional activator. Isoforms B have a shorter C-terminal part without complete TAD-B which acts as a transcriptional repressor.,domain:Rel Similarity Domain (RSD) allows DNA-binding and cooperative interactions with AP1 factors.,domain:The N-terminal transactivation domain (TAD-A) binds to and is activated by Cbp/p300. The dephosphorylated form contains two unmasked nuclear localization signals (NLS), which allow translocation o
Subcellular LocationCytoplasm . Nucleus . Cytoplasmic for the phosphorylated form and nuclear after activation that is controlled by calcineurin-mediated dephosphorylation. Rapid nuclear exit of NFATC is thought to be one mechanism by which cells distinguish between sustained and transient calcium signals. The subcellular localization of NFATC plays a key role in the regulation of gene transcription (PubMed:16511445). Nuclear translocation of NFATC1 is enhanced in the presence of TNFSF11. Nuclear translocation is decreased in the presence of FBN1 which can bind and sequester TNFSF11 (By similarity). .
ExpressionExpressed in thymus, peripheral leukocytes as T-cells and spleen. Isoforms A are preferentially expressed in effector T-cells (thymus and peripheral leukocytes) whereas isoforms B and isoforms C are preferentially expressed in naive T-cells (spleen). Isoforms B are expressed in naive T-cells after first antigen exposure and isoforms A are expressed in effector T-cells after second antigen exposure. Isoforms IA are widely expressed but not detected in liver nor pancreas, neural expression is strongest in corpus callosum. Isoforms IB are expressed mostly in muscle, cerebellum, placenta and thymus, neural expression in fetal and adult brain, strongest in corpus callosum.
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
ImmunogenThe antiserum was produced against synthesized peptide derived from the C-terminal region of human NFATC1. AA range:881-930
Gene NameNFATC1
DilutionWestern Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.
Backgroundnuclear factor of activated T-cells 1(NFATC1) Homo sapiens The product of this gene is a component of the nuclear factor of activated T cells DNA-binding transcription complex. This complex consists of at least two components: a preexisting cytosolic component that translocates to the nucleus upon T cell receptor (TCR) stimulation, and an inducible nuclear component. Proteins belonging to this family of transcription factors play a central role in inducible gene transcription during immune response. The product of this gene is an inducible nuclear component. It functions as a major molecular target for the immunosuppressive drugs such as cyclosporin A. Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. Different isoforms of this protein may regulate inducible expression of different cytokine genes. [provided by RefSeq, Jul 2013],
Conjugated Antibody
This product is also available conjugated. Format: E20-75381-Conjugate. Price: $485 / Size: 100μl.
E20-75381-FITC$485 / 100μlE20-75381-Biotin$485 / 100μlE20-75381-HRP$485 / 100μlE20-75381-AP$485 / 100μlE20-75381-AF350$485 / 100μlE20-75381-AF405M$485 / 100μlE20-75381-AF488$485 / 100μlE20-75381-AF514$485 / 100μlE20-75381-AF532$485 / 100μlE20-75381-AF555$485 / 100μlE20-75381-AF568$485 / 100μlE20-75381-AF405S$485 / 100μlE20-75381-AF405L$485 / 100μlE20-75381-AF546$485 / 100μlE20-75381-AF594$485 / 100μlE20-75381-AF610$485 / 100μlE20-75381-AF635$485 / 100μlE20-75381-AF647$485 / 100μlE20-75381-AF680$485 / 100μlE20-75381-AF700$485 / 100μlE20-75381-AF750$485 / 100μlE20-75381-AF790$485 / 100μlE20-75381-APC$485 / 100μlE20-75381-PE$485 / 100μlE20-75381-Cy3$485 / 100μlE20-75381-Cy5$485 / 100μlE20-75381-Cy5.5$485 / 100μlE20-75381-Cy7$485 / 100μlE20-75381-PE-Cy5$485 / 100μl
Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.
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