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Cell Signal Transduction Research Antibodies
Apaf-1 Polyclonal Antibody
Polyclonal, Rabbit
$225/ 100ul
QuantityTotal: $225
Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.
Product Information
Molecular Weight141840Da
Protein NameApoptotic protease-activating factor 1
Human Gene Id317
Human Swiss Prot NoO14727
Mouse Gene Id11783
Mouse Swiss Prot NoO88879
Rat Gene Id78963
Rat Swiss Prot NoQ9EPV5
SpecificityApaf-1 Polyclonal Antibody detects endogenous levels of Apaf-1 protein.
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration1 mg/ml
Storage Stability-20°C/1 year
Other NameAPAF1; KIAA0413; Apoptotic protease-activating factor 1; APAF-1
Observed Band(KD)135
Functiondomain:The CARD domain mediates interaction with APIP.,function:Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis.,induction:By E2F and p53 in apoptotic neurons.,similarity:Contains 1 CARD domain.,similarity:Contains 1 NB-ARC domain.,similarity:Contains 13 WD repeats.,subunit:Monomer. Oligomerizes upon binding of cytochrome c and dATP. Oligomeric Apaf-1 and pro-caspase-9 bind to each other via their respective NH2-terminal CARD domains and consecutively mature caspase-9 is released from the complex. Pro-caspase-3 is recruited into the Apaf-1-pro-caspase-9 complex via interaction with pro-caspase-9. Interacts with APIP.,tissue specificity:Ubiquitous. Highest levels of expression in adult spleen and per
Subcellular LocationCytoplasm .
ExpressionUbiquitous. Highest levels of expression in adult spleen and peripheral blood leukocytes, and in fetal brain, kidney and lung. Isoform 1 is expressed in heart, kidney and liver.
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
ImmunogenThe antiserum was produced against synthesized peptide derived from the Internal region of human APAF1. AA range:501-550
Gene NameAPAF1
DilutionWestern Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.
Backgroundapoptotic peptidase activating factor 1(APAF1) Homo sapiens This gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008],
Conjugated Antibody
This product is also available conjugated. Format: E20-75378-Conjugate. Price: $485 / Size: 100μl.
E20-75378-FITC$485 / 100μlE20-75378-Biotin$485 / 100μlE20-75378-HRP$485 / 100μlE20-75378-AP$485 / 100μlE20-75378-AF350$485 / 100μlE20-75378-AF405M$485 / 100μlE20-75378-AF488$485 / 100μlE20-75378-AF514$485 / 100μlE20-75378-AF532$485 / 100μlE20-75378-AF555$485 / 100μlE20-75378-AF568$485 / 100μlE20-75378-AF405S$485 / 100μlE20-75378-AF405L$485 / 100μlE20-75378-AF546$485 / 100μlE20-75378-AF594$485 / 100μlE20-75378-AF610$485 / 100μlE20-75378-AF635$485 / 100μlE20-75378-AF647$485 / 100μlE20-75378-AF680$485 / 100μlE20-75378-AF700$485 / 100μlE20-75378-AF750$485 / 100μlE20-75378-AF790$485 / 100μlE20-75378-APC$485 / 100μlE20-75378-PE$485 / 100μlE20-75378-Cy3$485 / 100μlE20-75378-Cy5$485 / 100μlE20-75378-Cy5.5$485 / 100μlE20-75378-Cy7$485 / 100μlE20-75378-PE-Cy5$485 / 100μl
Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.
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