HPA1 Polyclonal Antibody

Name: HPA1 Polyclonal Antibody
Brand: EnoGene Biotech Co. Ltd
SKU: E20-75353
Price: 225 USD
Availability: InStock

E20-75353Cite ID: EN0055486Datasheet COA MSDS

SourcePolyclonal, Rabbit

$225/ 100ul

Quantity

Total: $225

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

OverviewImages & Data (2)Documents

Product Information

Molecular Weight61177Da

Protein NameHeparanase

Human Gene Id10855

Human Swiss Prot NoQ9Y251

Mouse Swiss Prot NoQ6YGZ1

SpecificityHPA1 Polyclonal Antibody detects endogenous levels of HPA1 protein.

FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration1 mg/ml

Storage Stability-20°C/1 year

Other NameHPSE; HEP; HPA; HPA1; HPR1; HPSE1; HSE1; Heparanase; Endo-glucoronidase; Heparanase-1; Hpa1

Observed Band(KD)62

Functionenzyme regulation:Inhibited by EDTA, laminarin sulfate and, to a lower extent, by heparin and sulfamin and activated by calcium and magnesium.,function:Endoglycosidase which is a cell surface and extracellular matrix-degrading enzyme. Cleaves heparan sulfate proteoglycans (HSPGs) into heparan sulfate side chains and core proteoglycans. Also implicated in the extravasation of leukocytes and tumor cell lines. Due to its contribution to metastasis and angiogenesis, it is considered to be a potential target for anti-cancer therapies.,PTM:N-glycosylated. Glycosylation of the 50 kDa subunit appears to be essential for its solubility.,PTM:Proteolytically processed. The cleavage of the 65 kDa form leads to the generation of a linker peptide, 8 kDa and 50 kDa product. The active form, the 8/50 kDa heterodimer, is resistant to degradation. Complete removal of the linker peptide appears to be a pre

Subcellular LocationLysosome membrane; Peripheral membrane protein. Secreted. Nucleus. Proheparanase is secreted via vesicles of the Golgi. Interacts with cell membrane heparan sulfate proteoglycans (HSPGs). Endocytosed and accumulates in endosomes. Transferred to lysosomes where it is proteolytically cleaved to produce the active enzyme. Under certain stimuli, transferred to the cell surface. Associates with lipid rafts. Colocalizes with SDC1 in endosomal/lysosomal vesicles. Accumulates in perinuclear lysosomal vesicles. Heparin retains proheparanase in the extracellular medium (By similarity). .

ExpressionHighly expressed in placenta and spleen and weakly expressed in lymph node, thymus, peripheral blood leukocytes, bone marrow, endothelial cells, fetal liver and tumor tissues. Also expressed in hair follicles, specifically in both Henle's and Huxley's layers of inner the root sheath (IRS) at anagen phase.

PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

ImmunogenThe antiserum was produced against synthesized peptide derived from the Internal region of human HPSE. AA range:241-290

Gene NameHPSE

DilutionWestern Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.

Backgroundheparanase(HPSE) Homo sapiens Heparan sulfate proteoglycans are major components of the basement membrane and extracellular matrix. The protein encoded by this gene is an enzyme that cleaves heparan sulfate proteoglycans to permit cell movement through remodeling of the extracellular matrix. In addition, this cleavage can release bioactive molecules from the extracellular matrix. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011],

📄Datasheet
Full product datasheet (PDF)✅Certificate of Analysis
Lot-specific COA (generated from product data)🧪MSDS
Material safety data sheet (generated)

Conjugated Antibody

This product is also available conjugated. Format: E20-75353-Conjugate. Price: $485 / Size: 100μl.

E20-75353-FITC$485 / 100μlE20-75353-Biotin$485 / 100μlE20-75353-HRP$485 / 100μlE20-75353-AP$485 / 100μlE20-75353-AF350$485 / 100μlE20-75353-AF405M$485 / 100μlE20-75353-AF488$485 / 100μlE20-75353-AF514$485 / 100μlE20-75353-AF532$485 / 100μlE20-75353-AF555$485 / 100μlE20-75353-AF568$485 / 100μlE20-75353-AF405S$485 / 100μlE20-75353-AF405L$485 / 100μlE20-75353-AF546$485 / 100μlE20-75353-AF594$485 / 100μlE20-75353-AF610$485 / 100μlE20-75353-AF635$485 / 100μlE20-75353-AF647$485 / 100μlE20-75353-AF680$485 / 100μlE20-75353-AF700$485 / 100μlE20-75353-AF750$485 / 100μlE20-75353-AF790$485 / 100μlE20-75353-APC$485 / 100μlE20-75353-PE$485 / 100μlE20-75353-Cy3$485 / 100μlE20-75353-Cy5$485 / 100μlE20-75353-Cy5.5$485 / 100μlE20-75353-Cy7$485 / 100μlE20-75353-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.