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Cell Signal Transduction Research Antibodies
Btk Polyclonal Antibody
Polyclonal, Rabbit
$225/ 100ul
QuantityTotal: $225
Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.
Product Information
Molecular Weight76150Da
Protein NameTyrosine-protein kinase BTK
Human Gene Id695
Human Swiss Prot NoQ06187
Mouse Gene Id12229
Mouse Swiss Prot NoP35991
SpecificityBtk Polyclonal Antibody detects endogenous levels of Btk protein only when non-phosphorylation at Y550.
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration1 mg/ml
Storage Stability-20°C/1 year
Other NameBTK; AGMX1; ATK; BPK; Tyrosine-protein kinase BTK; Agammaglobulinaemia tyrosine kinase; ATK; B-cell progenitor kinase; BPK; Bruton tyrosine kinase
Observed Band(KD)76
Functioncatalytic activity:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.,cofactor:Binds 1 zinc ion per subunit.,disease:Defects in BTK are the cause of X-linked agammaglobulinemia (XLA) [MIM:300755]; also called X-linked agammaglobulinemia type 1 (AGMX1) or immunodeficiency type 1 (IMD1). XLA is a humoral immunodeficiency disease which results in developmental defects in the maturation pathway of B-cells. Affected boys have normal levels of pre-B-cells in their bone marrow but virtually no circulating mature B-lymphocytes. This results in a lack of immunoglobulins of all classes and leads to recurrent bacterial infections like otitis, conjunctivitis, dermatitis, sinusitis in the first few years of life, or even some patients present overwhelming sepsis or meningitis, resulting in death in a few hours. Treatment in most cases is by infusion of intravenous immunoglobulin.,
Subcellular LocationCytoplasm. Cell membrane; Peripheral membrane protein. Nucleus. In steady state, BTK is predominantly cytosolic. Following B-cell receptor (BCR) engagement by antigen, translocates to the plasma membrane through its PH domain. Plasma membrane localization is a critical step in the activation of BTK. A fraction of BTK also shuttles between the nucleus and the cytoplasm, and nuclear export is mediated by the nuclear export receptor CRM1.
ExpressionPredominantly expressed in B-lymphocytes.
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
ImmunogenSynthesized peptide derived from Btk . at AA range: 490-570
Gene NameBTK
DilutionWestern Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
BackgroundBruton tyrosine kinase(BTK) Homo sapiens The protein encoded by this gene plays a crucial role in B-cell development. Mutations in this gene cause X-linked agammaglobulinemia type 1, which is an immunodeficiency characterized by the failure to produce mature B lymphocytes, and associated with a failure of Ig heavy chain rearrangement. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2013],
Conjugated Antibody
This product is also available conjugated. Format: E20-75346-Conjugate. Price: $485 / Size: 100μl.
E20-75346-FITC$485 / 100μlE20-75346-Biotin$485 / 100μlE20-75346-HRP$485 / 100μlE20-75346-AP$485 / 100μlE20-75346-AF350$485 / 100μlE20-75346-AF405M$485 / 100μlE20-75346-AF488$485 / 100μlE20-75346-AF514$485 / 100μlE20-75346-AF532$485 / 100μlE20-75346-AF555$485 / 100μlE20-75346-AF568$485 / 100μlE20-75346-AF405S$485 / 100μlE20-75346-AF405L$485 / 100μlE20-75346-AF546$485 / 100μlE20-75346-AF594$485 / 100μlE20-75346-AF610$485 / 100μlE20-75346-AF635$485 / 100μlE20-75346-AF647$485 / 100μlE20-75346-AF680$485 / 100μlE20-75346-AF700$485 / 100μlE20-75346-AF750$485 / 100μlE20-75346-AF790$485 / 100μlE20-75346-APC$485 / 100μlE20-75346-PE$485 / 100μlE20-75346-Cy3$485 / 100μlE20-75346-Cy5$485 / 100μlE20-75346-Cy5.5$485 / 100μlE20-75346-Cy7$485 / 100μlE20-75346-PE-Cy5$485 / 100μl
Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.
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