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Cell Signal Transduction Research Antibodies
EPAS-1 Polyclonal Antibody
Polyclonal, Rabbit
$225/ 100ul
QuantityTotal: $225
Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.
Product Information
Molecular Weight96459Da
Protein NameEndothelial PAS domain-containing protein 1
Human Gene Id2034
Human Swiss Prot NoQ99814
Mouse Gene Id13819
Mouse Swiss Prot NoP97481
Rat Gene Id29452
Rat Swiss Prot NoQ9JHS1
SpecificityEPAS-1 Polyclonal Antibody detects endogenous levels of EPAS-1 protein.
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration1 mg/ml
Storage Stability-20°C/1 year
Other NameEPAS1; BHLHE73; HIF2A; MOP2; PASD2; Endothelial PAS domain-containing protein 1; EPAS-1; Basic-helix-loop-helix-PAS protein MOP2; Class E basic helix-loop-helix protein 73; bHLHe73;HIF-1-alpha-like factor; HLF; Hypoxia-inducible factor 2-alpha; HIF-2-alpha; HIF2-alpha; Member of PAS protein 2; PAS domain-containing protein 2
Observed Band(KD)96
Functiondisease:Defects in EPAS1 are the cause of erythrocytosis familial type 4 (ECYT4) [MIM:611783]. ECYT4 is an autosomal dominant disorder characterized by increased serum red blood cell mass, elevated hemoglobin concentration and hematocrit, and normal platelet and leukocyte counts.,function:Transcription factor involved in the induction of oxygen regulated genes. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier. Potent activator of the Tie-2 tyrosine kinase expression. Activation seems to require recruitment of transcriptional coactivators such as CREBPB and probably EP300
Subcellular LocationNucleus . Nucleus speckle . Colocalizes with HIF3A in the nucleus and speckles. .
ExpressionExpressed in most tissues, with highest levels in placenta, lung and heart. Selectively expressed in endothelial cells.
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
ImmunogenSynthesized peptide derived from human EPAS-1 around the non-acetylation site of K385.
Gene NameEPAS1 BHLHE73 HIF2A MOP2 PASD2
DilutionIF: 1:50-200 Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.
Backgroundendothelial PAS domain protein 1(EPAS1) Homo sapiens This gene encodes a transcription factor involved in the induction of genes regulated by oxygen, which is induced as oxygen levels fall. The encoded protein contains a basic-helix-loop-helix domain protein dimerization domain as well as a domain found in proteins in signal transduction pathways which respond to oxygen levels. Mutations in this gene are associated with erythrocytosis familial type 4. [provided by RefSeq, Nov 2009],
Conjugated Antibody
This product is also available conjugated. Format: E20-75325-Conjugate. Price: $485 / Size: 100μl.
E20-75325-FITC$485 / 100μlE20-75325-Biotin$485 / 100μlE20-75325-HRP$485 / 100μlE20-75325-AP$485 / 100μlE20-75325-AF350$485 / 100μlE20-75325-AF405M$485 / 100μlE20-75325-AF488$485 / 100μlE20-75325-AF514$485 / 100μlE20-75325-AF532$485 / 100μlE20-75325-AF555$485 / 100μlE20-75325-AF568$485 / 100μlE20-75325-AF405S$485 / 100μlE20-75325-AF405L$485 / 100μlE20-75325-AF546$485 / 100μlE20-75325-AF594$485 / 100μlE20-75325-AF610$485 / 100μlE20-75325-AF635$485 / 100μlE20-75325-AF647$485 / 100μlE20-75325-AF680$485 / 100μlE20-75325-AF700$485 / 100μlE20-75325-AF750$485 / 100μlE20-75325-AF790$485 / 100μlE20-75325-APC$485 / 100μlE20-75325-PE$485 / 100μlE20-75325-Cy3$485 / 100μlE20-75325-Cy5$485 / 100μlE20-75325-Cy5.5$485 / 100μlE20-75325-Cy7$485 / 100μlE20-75325-PE-Cy5$485 / 100μl
Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.
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