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Cell Signal Transduction Research Antibodies
Amphiphysin II Polyclonal Antibody
Polyclonal, Rabbit
$225/ 100ul
QuantityTotal: $225
Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.
Product Information
Molecular Weight64699Da
Protein NameMyc box-dependent-interacting protein 1
Human Gene Id274
Human Swiss Prot NoO00499
Mouse Gene Id30948
Mouse Swiss Prot NoO08539
Rat Gene Id117028
Rat Swiss Prot NoO08839
SpecificityAmphiphysin II Polyclonal Antibody detects endogenous levels of Amphiphysin II protein.
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration1 mg/ml
Storage Stability-20°C/1 year
Other NameBIN1; AMPHL; Myc box-dependent-interacting protein 1; Amphiphysin II; Amphiphysin-like protein; Box-dependent myc-interacting protein 1; Bridging integrator 1
Observed Band(KD)64
Functionalternative products:Additional isoforms seem to exist,disease:Defects in BIN1 are the cause of centronuclear myopathy autosomal recessive (ARCNM) [MIM:255200]; also known as autosomal recessive myotubular myopathy. Centronuclear myopathies are congenital muscle disorders characterized by progressive muscular weakness and wasting involving mainly limb girdle, trunk, and neck muscles. It may also affect distal muscles. Weakness may be present during childhood or adolescence or may not become evident until the third decade of life. Ptosis is a frequent clinical feature. The most prominent histopathologic features include high frequency of centrally located nuclei in muscle fibers not secondary to regeneration, radial arrangement of sarcoplasmic strands around the central nuclei, and predominance and hypotrophy of type 1 fibers.,function:May be involved in regulation of synaptic vesicle end
Subcellular Location[Isoform BIN1]: Nucleus . Cytoplasm . Endosome . Cell membrane, sarcolemma, T-tubule .; [Isoform IIA]: Cytoplasm .
ExpressionUbiquitous. Highest expression in the brain and muscle (PubMed:9182667). Expressed in oligodendrocytes (PubMed:27488240). Isoform IIA is expressed only in the brain, where it is detected in the gray matter, but not in the white matter (PubMed:27488240). Isoform BIN1 is widely expressed with highest expression in skeletal muscle.
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
ImmunogenSynthesized peptide derived from the C-terminal region of human Amphiphysin II.
Gene NameBIN1
DilutionWestern Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.
Backgroundbridging integrator 1(BIN1) Homo sapiens This gene encodes several isoforms of a nucleocytoplasmic adaptor protein, one of which was initially identified as a MYC-interacting protein with features of a tumor suppressor. Isoforms that are expressed in the central nervous system may be involved in synaptic vesicle endocytosis and may interact with dynamin, synaptojanin, endophilin, and clathrin. Isoforms that are expressed in muscle and ubiquitously expressed isoforms localize to the cytoplasm and nucleus and activate a caspase-independent apoptotic process. Studies in mouse suggest that this gene plays an important role in cardiac muscle development. Alternate splicing of the gene results in several transcript variants encoding different isoforms. Aberrant splice variants expressed in tumor cell lines have also been described. [provided by RefSeq, Mar 2016],
Conjugated Antibody
This product is also available conjugated. Format: E20-75316-Conjugate. Price: $485 / Size: 100μl.
E20-75316-FITC$485 / 100μlE20-75316-Biotin$485 / 100μlE20-75316-HRP$485 / 100μlE20-75316-AP$485 / 100μlE20-75316-AF350$485 / 100μlE20-75316-AF405M$485 / 100μlE20-75316-AF488$485 / 100μlE20-75316-AF514$485 / 100μlE20-75316-AF532$485 / 100μlE20-75316-AF555$485 / 100μlE20-75316-AF568$485 / 100μlE20-75316-AF405S$485 / 100μlE20-75316-AF405L$485 / 100μlE20-75316-AF546$485 / 100μlE20-75316-AF594$485 / 100μlE20-75316-AF610$485 / 100μlE20-75316-AF635$485 / 100μlE20-75316-AF647$485 / 100μlE20-75316-AF680$485 / 100μlE20-75316-AF700$485 / 100μlE20-75316-AF750$485 / 100μlE20-75316-AF790$485 / 100μlE20-75316-APC$485 / 100μlE20-75316-PE$485 / 100μlE20-75316-Cy3$485 / 100μlE20-75316-Cy5$485 / 100μlE20-75316-Cy5.5$485 / 100μlE20-75316-Cy7$485 / 100μlE20-75316-PE-Cy5$485 / 100μl
Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.
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