CD177 Polyclonal Antibody

Name: CD177 Polyclonal Antibody
Brand: EnoGene Biotech Co. Ltd
SKU: E20-75283
Price: 225 USD
Availability: InStock

E20-75283Cite ID: EN0055416Datasheet COA MSDS

SourcePolyclonal, Rabbit

$225/ 100ul

Quantity

Total: $225

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

OverviewImages & Data (2)Documents

Product Information

Molecular Weight46363Da

Protein NameCD177 antigen

Human Gene Id57126

Human Swiss Prot NoQ8N6Q3

Mouse Gene Id68891

Mouse Swiss Prot NoQ8R2S8

SpecificityCD177 Polyclonal Antibody detects endogenous levels of CD177 protein.

FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration1 mg/ml

Storage Stability-20°C/1 year

Other NameCD177; NB1; PRV1; CD177 antigen; Human neutrophil alloantigen 2a; HNA-2a; NB1 glycoprotein; NB1 GP; Polycythemia rubra vera protein 1; PRV-1; CD177

Observed Band(KD)46

Functioninduction:By CSF3 in resting granulocytes. Induced in patients with polycythemia vera (PV) and with essential thrombocythemia (ET).,mass spectrometry: PubMed:11465086,polymorphism:There is a significant association between the variants Ala-3, Leu-251 and Thr-348 and a low expression of CD177 on neutrophils.,PTM:A soluble form may also be produced by proteolytic cleavage at the cell surface (shedding).,PTM:N-glycosylated.,similarity:Contains 2 UPAR/Ly6 domains.,tissue specificity:Highly expressed in normal bone marrow and weakly expressed in fetal liver. Expressed on neutrophils. Expressed in granulocytes of patients with polycythemia vera (PV) and with essential thrombocythemia (ET).,

Subcellular LocationCell membrane ; Lipid-anchor, GPI-anchor . Membrane raft ; Lipid-anchor, GPI-like-anchor . Secreted . Cytoplasmic granule membrane . Cell projection, lamellipodium . Cell surface expression on neutrophils is increased upon TNF-alpha, fMLP or CXCL8/IL8-mediated stimulation (PubMed:17244676, PubMed:17580308). In neutrophils, stored predominantly in secondary and tertiary granules (PubMed:18462208). Can also be shedded from the cell membrane (PubMed:12239154, PubMed:18462208). Localizes to lamellar protrusions in spreading neutrophils (PubMed:28807980). .

ExpressionHighly expressed in normal bone marrow and weakly expressed in fetal liver (PubMed:10753836). During neutrophil differentiation, expression begins at the metamyelocyte stage and continues throughout the subsequent stages (at protein level) (PubMed:17244676, PubMed:18462208, PubMed:24926686). Expressed by a subset of mature neutrophils (at protein level) (PubMed:10753836, PubMed:28240246, PubMed:12377969, PubMed:18462208, PubMed:12675722, PubMed:17244676, PubMed:17580308, PubMed:21193407, PubMed:24926686, PubMed:28807980, PubMed:27227454). The percentage of neutrophils expressing CD177 varies across the population (PubMed:17244676, PubMed:27227454). Expressed in granulocytes of patients with polycythemia vera (PV) and with essential thrombocythemia (ET) (PubMed:10753836, PubMed:12377969).

PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

ImmunogenThe antiserum was produced against synthesized peptide derived from the Internal region of human CD177. AA range:361-410

Gene NameCD177

DilutionWestern Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.

BackgroundCD177 molecule(CD177) Homo sapiens This gene encodes a glycosyl-phosphatidylinositol (GPI)-linked cell surface glycoprotein that plays a role in neutrophil activation. The protein can bind platelet endothelial cell adhesion molecule-1 and function in neutrophil transmigration. Mutations in this gene are associated with myeloproliferative diseases. Over-expression of this gene has been found in patients with polycythemia rubra vera. Autoantibodies against the protein may result in pulmonary transfusion reactions, and it may be involved in Wegener's granulomatosis. A related pseudogene, which is adjacent to this gene on chromosome 19, has been identified. [provided by RefSeq, Apr 2014],

📄Datasheet
Full product datasheet (PDF)✅Certificate of Analysis
Lot-specific COA (generated from product data)🧪MSDS
Material safety data sheet (generated)

Conjugated Antibody

This product is also available conjugated. Format: E20-75283-Conjugate. Price: $485 / Size: 100μl.

E20-75283-FITC$485 / 100μlE20-75283-Biotin$485 / 100μlE20-75283-HRP$485 / 100μlE20-75283-AP$485 / 100μlE20-75283-AF350$485 / 100μlE20-75283-AF405M$485 / 100μlE20-75283-AF488$485 / 100μlE20-75283-AF514$485 / 100μlE20-75283-AF532$485 / 100μlE20-75283-AF555$485 / 100μlE20-75283-AF568$485 / 100μlE20-75283-AF405S$485 / 100μlE20-75283-AF405L$485 / 100μlE20-75283-AF546$485 / 100μlE20-75283-AF594$485 / 100μlE20-75283-AF610$485 / 100μlE20-75283-AF635$485 / 100μlE20-75283-AF647$485 / 100μlE20-75283-AF680$485 / 100μlE20-75283-AF700$485 / 100μlE20-75283-AF750$485 / 100μlE20-75283-AF790$485 / 100μlE20-75283-APC$485 / 100μlE20-75283-PE$485 / 100μlE20-75283-Cy3$485 / 100μlE20-75283-Cy5$485 / 100μlE20-75283-Cy5.5$485 / 100μlE20-75283-Cy7$485 / 100μlE20-75283-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.