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Cell Signal Transduction Research Antibodies
Ob-R Polyclonal Antibody
Polyclonal, Rabbit
$225/ 100ul
QuantityTotal: $225
Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.
Product Information
Molecular Weight132494Da
Protein NameLeptin receptor
Human Gene Id3953
Human Swiss Prot NoP48357
Mouse Gene Id16847
Mouse Swiss Prot NoP48356
Rat Gene Id24536
Rat Swiss Prot NoQ62959
SpecificityOb-R Polyclonal Antibody detects endogenous levels of Ob-R protein.
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration1 mg/ml
Storage Stability-20°C/1 year
Other NameLEPR; DB; OBR; Leptin receptor; LEP-R; HuB219; OB receptor; OB-R; CD295
Observed Band(KD)132
Functiondomain:The box 1 motif is required for JAK interaction and/or activation.,domain:The cytoplasmic domain may be essential for intracellular signal transduction by activation of JAK tyrosine kinase and STATs.,domain:The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.,function:Receptor for obesity factor (leptin). On ligand binding, mediates signaling through JAK2/STAT3. Involved in the regulation of fat metabolism and, in a hematopoietic pathway, required for normal lymphopoiesis. May play a role in reproduction. Can also mediate the ERK/FOS signaling pathway.,PTM:On ligand binding, phosphorylated on two conserved C-terminal tyrosine residues (isoform B only) by JAK2. Tyr-986 is required for complete binding and activation of PTPN11, ERK/FOS activation and, for interaction with SOCS3 (By similar
Subcellular LocationCell membrane ; Single-pass type I membrane protein . Basolateral cell membrane .; [Isoform E]: Secreted .
ExpressionIsoform A is expressed in fetal liver and in hematopoietic tissues and choroid plexus. In adults highest expression in heart, liver, small intestine, prostate and ovary. Low level in lung and kidney. Isoform B is highly expressed in hypothalamus, but also in skeletal muscle. Detected in fundic and antral epithelial cells of the gastric mucosa (PubMed:19159218). Isoform B and isoform A are expressed by NK cells (at protein level) (PubMed:12504075).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
ImmunogenSynthesized peptide derived from Leptin receptor at AA range: 791-840
Gene NameLEPR OBR
DilutionWestern Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Backgroundleptin receptor(LEPR) Homo sapiens The protein encoded by this gene belongs to the gp130 family of cytokine receptors that are known to stimulate gene transcription via activation of cytosolic STAT proteins. This protein is a receptor for leptin (an adipocyte-specific hormone that regulates body weight), and is involved in the regulation of fat metabolism, as well as in a novel hematopoietic pathway that is required for normal lymphopoiesis. Mutations in this gene have been associated with obesity and pituitary dysfunction. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. It is noteworthy that this gene and LEPROT gene (GeneID:54741) share the same promoter and the first 2 exons, however, encode distinct proteins (PMID:9207021).[provided by RefSeq, Nov 2010],
Conjugated Antibody
This product is also available conjugated. Format: E20-75282-Conjugate. Price: $485 / Size: 100μl.
E20-75282-FITC$485 / 100μlE20-75282-Biotin$485 / 100μlE20-75282-HRP$485 / 100μlE20-75282-AP$485 / 100μlE20-75282-AF350$485 / 100μlE20-75282-AF405M$485 / 100μlE20-75282-AF488$485 / 100μlE20-75282-AF514$485 / 100μlE20-75282-AF532$485 / 100μlE20-75282-AF555$485 / 100μlE20-75282-AF568$485 / 100μlE20-75282-AF405S$485 / 100μlE20-75282-AF405L$485 / 100μlE20-75282-AF546$485 / 100μlE20-75282-AF594$485 / 100μlE20-75282-AF610$485 / 100μlE20-75282-AF635$485 / 100μlE20-75282-AF647$485 / 100μlE20-75282-AF680$485 / 100μlE20-75282-AF700$485 / 100μlE20-75282-AF750$485 / 100μlE20-75282-AF790$485 / 100μlE20-75282-APC$485 / 100μlE20-75282-PE$485 / 100μlE20-75282-Cy3$485 / 100μlE20-75282-Cy5$485 / 100μlE20-75282-Cy5.5$485 / 100μlE20-75282-Cy7$485 / 100μlE20-75282-PE-Cy5$485 / 100μl
Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.
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