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Cell Signal Transduction Research Antibodies
Keap1 Polyclonal Antibody
Polyclonal, Rabbit
$225/ 100ul
QuantityTotal: $225
Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.
Product Information
Molecular Weight68640Da
Protein NameKelch-like ECH-associated protein 1
Human Gene Id9817
Human Swiss Prot NoQ14145
Mouse Gene Id50868
Mouse Swiss Prot NoQ9Z2X8
Rat Gene Id117519
Rat Swiss Prot NoP57790
SpecificityKeap1 Polyclonal Antibody detects endogenous levels of Keap1 protein.
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration1 mg/ml
Storage Stability-20°C/1 year
Other NameKEAP1; INRF2; KIAA0132; KLHL19; Kelch-like ECH-associated protein 1; Cytosolic inhibitor of Nrf2; INrf2; Kelch-like protein 19
Observed Band(KD)70
Functiondisease:Defects in KEAP1 may be a cause of breast cancer.,disease:Defects in KEAP1 may be involved in non small cell lung carcinomas (NSCLC) and lung adenocarcinoma.,domain:The Kelch repeats mediate interaction with NF2L2/NRF2, BPTF and PGAM5.,enzyme regulation:Ubiquitination and subsequent degradation of PGAM5 is inhibited by oxidative stress and sulforaphane.,function:Retains NFE2L2/NRF2 in the cytosol. Functions as substrate adapter protein for the E3 ubiquitin ligase complex formed by CUL3 and RBX1. Targets NFE2L2/NRF2 for ubiquitination and degradation by the proteasome, thus resulting in the suppression of its transcriptional activity and the repression of antioxidant response element-mediated detoxifying enzyme gene expression. May also retain BPTF in the cytosol. Targets PGAM5 for ubiquitination and degradation by the proteasome.,PTM:Ubiquitinated and subject to proteasomal degra
Subcellular LocationCytoplasm . Nucleus . Mainly cytoplasmic (PubMed:15601839). In response to selective autophagy, relocalizes to inclusion bodies following interaction with SQSTM1/p62 (PubMed:20452972). .
ExpressionBroadly expressed, with highest levels in skeletal muscle.
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
ImmunogenThe antiserum was produced against synthesized peptide derived from the Internal region of human KEAP1. AA range:411-460
Gene NameKEAP1
DilutionWestern Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.
Backgroundkelch like ECH associated protein 1(KEAP1) Homo sapiens This gene encodes a protein containing KELCH-1 like domains, as well as a BTB/POZ domain. Kelch-like ECH-associated protein 1 interacts with NF-E2-related factor 2 in a redox-sensitive manner and the dissociation of the proteins in the cytoplasm is followed by transportation of NF-E2-related factor 2 to the nucleus. This interaction results in the expression of the catalytic subunit of gamma-glutamylcysteine synthetase. Two alternatively spliced transcript variants encoding the same isoform have been found for this gene. [provided by RefSeq, Jul 2008],
Conjugated Antibody
This product is also available conjugated. Format: E20-75218-Conjugate. Price: $485 / Size: 100μl.
E20-75218-FITC$485 / 100μlE20-75218-Biotin$485 / 100μlE20-75218-HRP$485 / 100μlE20-75218-AP$485 / 100μlE20-75218-AF350$485 / 100μlE20-75218-AF405M$485 / 100μlE20-75218-AF488$485 / 100μlE20-75218-AF514$485 / 100μlE20-75218-AF532$485 / 100μlE20-75218-AF555$485 / 100μlE20-75218-AF568$485 / 100μlE20-75218-AF405S$485 / 100μlE20-75218-AF405L$485 / 100μlE20-75218-AF546$485 / 100μlE20-75218-AF594$485 / 100μlE20-75218-AF610$485 / 100μlE20-75218-AF635$485 / 100μlE20-75218-AF647$485 / 100μlE20-75218-AF680$485 / 100μlE20-75218-AF700$485 / 100μlE20-75218-AF750$485 / 100μlE20-75218-AF790$485 / 100μlE20-75218-APC$485 / 100μlE20-75218-PE$485 / 100μlE20-75218-Cy3$485 / 100μlE20-75218-Cy5$485 / 100μlE20-75218-Cy5.5$485 / 100μlE20-75218-Cy7$485 / 100μlE20-75218-PE-Cy5$485 / 100μl
Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.
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