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Cell Signal Transduction Research Antibodies
Caspase-3 Polyclonal Antibody
Polyclonal, Rabbit
$225/ 100ul
QuantityTotal: $225
Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.
Product Information
Molecular Weight31608Da
Protein NameCaspase3
Human Gene Id836
Human Swiss Prot NoP42574
Mouse Gene Id12367
Mouse Swiss Prot NoP70677
Rat Gene Id25402
Rat Swiss Prot NoP55213
SpecificityCaspase-3 Polyclonal Antibody detects endogenous levels of Caspase-3 protein.
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration1 mg/ml
Storage Stability-20°C/1 year
Other NameCASP3; CPP32; Caspase-3; CASP-3; Apopain; Cysteine protease CPP32; CPP-32; Protein Yama; SREBP cleavage activity 1; SCA-1
Observed Band(KD)35
Functioncatalytic activity:Strict requirement for an Asp residue at positions P1 and P4. It has a preferred cleavage sequence of Asp-Xaa-Xaa-Asp-|- with a hydrophobic amino-acid residue at P2 and a hydrophilic amino-acid residue at P3, although Val or Ala are also accepted at this position.,enzyme regulation:Inhibited by isatin sulfonamides.,function:Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin.,PTM:Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Ad
Subcellular LocationCytoplasm.
ExpressionHighly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
ImmunogenThe antiserum was produced against synthesized peptide derived from the Internal region of human CASP3. AA range:181-230
Gene NameCASP3
DilutionIF: 1:50-200 WB 1:500-2000, ELISA 1:10000-20000 IHC 1:50-300
Backgroundcaspase 3(CASP3) Homo sapiens This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein. [provided by RefSeq, Jul 2008],
Conjugated Antibody
This product is also available conjugated. Format: E20-75204-Conjugate. Price: $485 / Size: 100μl.
E20-75204-FITC$485 / 100μlE20-75204-Biotin$485 / 100μlE20-75204-HRP$485 / 100μlE20-75204-AP$485 / 100μlE20-75204-AF350$485 / 100μlE20-75204-AF405M$485 / 100μlE20-75204-AF488$485 / 100μlE20-75204-AF514$485 / 100μlE20-75204-AF532$485 / 100μlE20-75204-AF555$485 / 100μlE20-75204-AF568$485 / 100μlE20-75204-AF405S$485 / 100μlE20-75204-AF405L$485 / 100μlE20-75204-AF546$485 / 100μlE20-75204-AF594$485 / 100μlE20-75204-AF610$485 / 100μlE20-75204-AF635$485 / 100μlE20-75204-AF647$485 / 100μlE20-75204-AF680$485 / 100μlE20-75204-AF700$485 / 100μlE20-75204-AF750$485 / 100μlE20-75204-AF790$485 / 100μlE20-75204-APC$485 / 100μlE20-75204-PE$485 / 100μlE20-75204-Cy3$485 / 100μlE20-75204-Cy5$485 / 100μlE20-75204-Cy5.5$485 / 100μlE20-75204-Cy7$485 / 100μlE20-75204-PE-Cy5$485 / 100μl
Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.
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