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Cell Signal Transduction Research Antibodies
IL-1β Polyclonal Antibody
Polyclonal, Rabbit
$225/ 100ul
QuantityTotal: $225
Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.
Product Information
Molecular Weight30748Da
Protein NameInterleukin-1 beta
Human Gene Id3553
Human Swiss Prot NoP01584
Mouse Gene Id16176
Mouse Swiss Prot NoP10749
Rat Swiss Prot NoQ63264
SpecificityIL-1β Polyclonal Antibody detects endogenous levels of IL-1β protein.
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration1 mg/ml
Storage Stability-20°C/1 year
Other NameIL1B; IL1F2; Interleukin-1 beta; IL-1 beta; Catabolin
Observed Band(KD)17
Functiondomain:The similarity among the IL-1 precursors suggests that the amino ends of these proteins serve some as yet undefined function.,function:Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.,online information:Interleukin-1 entry,online information:The Singapore human mutation and polymorphism database,similarity:Belongs to the IL-1 family.,subcellular location:The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.,subunit:Mono
Subcellular LocationCytoplasm, cytosol . Secreted . Lysosome . Secreted, extracellular exosome . The precursor is cytosolic (PubMed:15192144). In response to inflammasome-activating signals, such as ATP for NLRP3 inflammasome or bacterial flagellin for NLRC4 inflammasome, cleaved and secreted (PubMed:24201029, PubMed:33377178, PubMed:33883744). Mature form is secreted and released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore (PubMed:33883744). In contrast, the precursor form is not released, due to the presence of an acidic region that is proteolytically removed by CASP1 during maturation (PubMed:33883744). The secretion is dependent on protein unfolding and facilitated by the cargo receptor TMED10 (PubMed:32272059). .
ExpressionExpressed in activated monocytes/macrophages (at protein level).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
ImmunogenThe antiserum was produced against synthesized peptide derived from the Internal region of human IL1B. AA range:181-230
Gene NameIL1B
DilutionWestern Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. IF 1:100-300 Not yet tested in other applications.
Backgroundinterleukin 1 beta(IL1B) Homo sapiens The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. [provided by RefSeq, Jul 2008],
Conjugated Antibody
This product is also available conjugated. Format: E20-75201-Conjugate. Price: $485 / Size: 100μl.
E20-75201-FITC$485 / 100μlE20-75201-Biotin$485 / 100μlE20-75201-HRP$485 / 100μlE20-75201-AP$485 / 100μlE20-75201-AF350$485 / 100μlE20-75201-AF405M$485 / 100μlE20-75201-AF488$485 / 100μlE20-75201-AF514$485 / 100μlE20-75201-AF532$485 / 100μlE20-75201-AF555$485 / 100μlE20-75201-AF568$485 / 100μlE20-75201-AF405S$485 / 100μlE20-75201-AF405L$485 / 100μlE20-75201-AF546$485 / 100μlE20-75201-AF594$485 / 100μlE20-75201-AF610$485 / 100μlE20-75201-AF635$485 / 100μlE20-75201-AF647$485 / 100μlE20-75201-AF680$485 / 100μlE20-75201-AF700$485 / 100μlE20-75201-AF750$485 / 100μlE20-75201-AF790$485 / 100μlE20-75201-APC$485 / 100μlE20-75201-PE$485 / 100μlE20-75201-Cy3$485 / 100μlE20-75201-Cy5$485 / 100μlE20-75201-Cy5.5$485 / 100μlE20-75201-Cy7$485 / 100μlE20-75201-PE-Cy5$485 / 100μl
Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.
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