Cdk4 Polyclonal Antibody

Name: Cdk4 Polyclonal Antibody
Brand: EnoGene Biotech Co. Ltd
SKU: E20-75198
Price: 225 USD
Availability: InStock

E20-75198Cite ID: EN0055331Datasheet COA MSDS

Source： Polyclonal, Rabbit

$225/ 100ul

Quantity

Total: $225

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

OverviewImages & Data (2)Documents

Product Information

Molecular Weight： 33730Da

Protein Name： Cyclin-dependent kinase 4

Human Gene Id： 1019

Human Swiss Prot No： P11802

Mouse Gene Id： 12567

Mouse Swiss Prot No： P30285

Rat Gene Id： 94201

Rat Swiss Prot No： P35426

Specificity： Cdk4 Polyclonal Antibody detects endogenous levels of Cdk4 protein.

Formulation： Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration： 1 mg/ml

Storage Stability： -20°C/1 year

Other Name： CDK4; Cyclin-dependent kinase 4; Cell division protein kinase 4; PSK-J3

Observed Band(KD)： 35

Function： catalytic activity:ATP + a protein = ADP + a phosphoprotein.,disease:CDK4 mutations are involved in tumor formation.,disease:Defects in CDK4 are the cause of cutaneous malignant melanoma 3 (CMM3) [MIM:609048, 155600]. Malignant melanoma is a malignant neoplasm of melanocytes, arising de novo or from a preexisting benign nevus, which occurs most often in the skin but also may involve other sites.,enzyme regulation:Phosphorylation at Thr-172 is necessary for enzymatic activity.,function:Probably involved in the control of the cell cycle.,similarity:Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.,similarity:Contains 1 protein kinase domain.,subunit:Forms a stable complex with D-type G1 cyclins. Interacts with SEI1 and ZNF655/VIK.,

Subcellular Location： Cytoplasm . Nucleus . Nucleus membrane . Cytoplasmic when non-complexed. Forms a cyclin D-CDK4 complex in the cytoplasm as cells progress through G(1) phase. The complex accumulates on the nuclear membrane and enters the nucleus on transition from G(1) to S phase. Also present in nucleoli and heterochromatin lumps. Colocalizes with RB1 after release into the nucleus. .

Expression： Brain,Muscle,

Purification： The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen： The antiserum was produced against synthesized peptide derived from the N-terminal region of human CDK4. AA range:1-50

Gene Name： CDK4

Dilution： IF: 1:50-200 Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.

Background： cyclin dependent kinase 4(CDK4) Homo sapiens The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is highly similar to the gene products of S. cerevisiae cdc28 and S. pombe cdc2. It is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of this kinase is restricted to the G1-S phase, which is controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16(INK4a). This kinase was shown to be responsible for the phosphorylation of retinoblastoma gene product (Rb). Mutations in this gene as well as in its related proteins including D-type cyclins, p16(INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers. Multiple polyadenylation sites of this gene have been reported. [provided by RefSeq, Jul 2008],

📄Datasheet
Full product datasheet (PDF)✅Certificate of Analysis
Lot-specific COA (generated from product data)🧪MSDS
Material safety data sheet (generated)

Conjugated Antibody

This product is also available conjugated. Format: E20-75198-Conjugate. Price: $485 / Size: 100μl.

E20-75198-FITC$485 / 100μlE20-75198-Biotin$485 / 100μlE20-75198-HRP$485 / 100μlE20-75198-AP$485 / 100μlE20-75198-AF350$485 / 100μlE20-75198-AF405M$485 / 100μlE20-75198-AF488$485 / 100μlE20-75198-AF514$485 / 100μlE20-75198-AF532$485 / 100μlE20-75198-AF555$485 / 100μlE20-75198-AF568$485 / 100μlE20-75198-AF405S$485 / 100μlE20-75198-AF405L$485 / 100μlE20-75198-AF546$485 / 100μlE20-75198-AF594$485 / 100μlE20-75198-AF610$485 / 100μlE20-75198-AF635$485 / 100μlE20-75198-AF647$485 / 100μlE20-75198-AF680$485 / 100μlE20-75198-AF700$485 / 100μlE20-75198-AF750$485 / 100μlE20-75198-AF790$485 / 100μlE20-75198-APC$485 / 100μlE20-75198-PE$485 / 100μlE20-75198-Cy3$485 / 100μlE20-75198-Cy5$485 / 100μlE20-75198-Cy5.5$485 / 100μlE20-75198-Cy7$485 / 100μlE20-75198-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.