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Cell Signal Transduction Research Antibodies
AFP Polyclonal Antibody
Polyclonal, Rabbit
$225/ 100ul
QuantityTotal: $225
Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.
Product Information
Molecular Weight68678DaObserved Band:70KD
Protein NameAlpha-fetoprotein
Human Gene Id174
Human Swiss Prot NoP02771
Mouse Swiss Prot NoP02772
SpecificityAFP Polyclonal Antibody detects endogenous levels of AFP protein.
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration1 mg/ml
Storage Stability-20°C/1 year
Other NameAFP; HPAFP; Alpha-fetoprotein; Alpha-1-fetoprotein; Alpha-fetoglobulin
Functiondevelopmental stage:Occurs in the plasma of fetuses more than 4 weeks old, reaches the highest levels during the 12th-16th week of gestation, and drops to trace amounts after birth. The serum level in adults is usually less than 40 ng/ml. AFP occurs also at high levels in the plasma and ascitic fluid of adults with hepatoma.,function:Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.,online information:Alpha-fetoprotein entry,PTM:Independent studies suggest heterogeneity of the N-terminal sequence of the mature protein and of the cleavage site of the signal sequence.,PTM:Sulfated.,similarity:Belongs to the ALB/AFP/VDB family.,similarity:Contains 3 albumin domains.,subunit:Dimeric and trimeric forms have been found in addition to the monomeric form.,tissue
Subcellular LocationSecreted.
ExpressionPlasma. Synthesized by the fetal liver and yolk sac.
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
ImmunogenThe antiserum was produced against synthesized peptide derived from the Internal region of human AFP. AA range:371-420
Gene NameAFP
DilutionWestern Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.
Backgroundalpha fetoprotein(AFP) Homo sapiens This gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. [provided by RefSeq, Jul 2008],
Conjugated Antibody
This product is also available conjugated. Format: E20-75140-Conjugate. Price: $485 / Size: 100μl.
E20-75140-FITC$485 / 100μlE20-75140-Biotin$485 / 100μlE20-75140-HRP$485 / 100μlE20-75140-AP$485 / 100μlE20-75140-AF350$485 / 100μlE20-75140-AF405M$485 / 100μlE20-75140-AF488$485 / 100μlE20-75140-AF514$485 / 100μlE20-75140-AF532$485 / 100μlE20-75140-AF555$485 / 100μlE20-75140-AF568$485 / 100μlE20-75140-AF405S$485 / 100μlE20-75140-AF405L$485 / 100μlE20-75140-AF546$485 / 100μlE20-75140-AF594$485 / 100μlE20-75140-AF610$485 / 100μlE20-75140-AF635$485 / 100μlE20-75140-AF647$485 / 100μlE20-75140-AF680$485 / 100μlE20-75140-AF700$485 / 100μlE20-75140-AF750$485 / 100μlE20-75140-AF790$485 / 100μlE20-75140-APC$485 / 100μlE20-75140-PE$485 / 100μlE20-75140-Cy3$485 / 100μlE20-75140-Cy5$485 / 100μlE20-75140-Cy5.5$485 / 100μlE20-75140-Cy7$485 / 100μlE20-75140-PE-Cy5$485 / 100μl
Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.
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