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Cell Signal Transduction Research Antibodies
Smad5 Polyclonal Antibody
Polyclonal, Rabbit
$225/ 100ul
QuantityTotal: $225
Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.
Product Information
Molecular Weight52258Da
Protein NameMothers against decapentaplegic homolog 5
Human Gene Id4090
Human Swiss Prot NoQ99717
Mouse Swiss Prot NoP97454
Rat Gene Id59328
Rat Swiss Prot NoQ9R1V3
SpecificitySmad5 Polyclonal Antibody detects endogenous levels of Smad5 protein.
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration1 mg/ml
Storage Stability-20°C/1 year
Other NameSMAD5; MADH5; Mothers against decapentaplegic homolog 5; MAD homolog 5; Mothers against DPP homolog 5; JV5-1; SMAD family member 5; SMAD 5; Smad5; hSmad5
Observed Band(KD)52
Functionfunction:Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD).,PTM:Phosphorylated on serine by BMP (bone morphogenetic proteins) type 1 receptor kinase.,PTM:Ubiquitin-mediated proteolysis by SMAD-specific E3 ubiquitin ligase SMURF1.,similarity:Belongs to the dwarfin/SMAD family.,similarity:Contains 1 MH1 (MAD homology 1) domain.,similarity:Contains 1 MH2 (MAD homology 2) domain.,subcellular location:Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4.,subunit:May form trimers with the co-SMAD SMAD4. Interacts with PEBP2-alpha subunit and SMURF1. Interacts with SUV39H1 and SUV39H2.,tissue specificity:Ubiquitous.,
Subcellular LocationCytoplasm. Nucleus. Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4.
ExpressionUbiquitous.
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
ImmunogenSynthesized peptide derived from the Internal region of human Smad5.
Gene NameSMAD5
DilutionWestern Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/40000. Not yet tested in other applications.
BackgroundSMAD family member 5(SMAD5) Homo sapiens The protein encoded by this gene is involved in the transforming growth factor beta signaling pathway that results in an inhibition of the proliferation of hematopoietic progenitor cells. The encoded protein is activated by bone morphogenetic proteins type 1 receptor kinase, and may be involved in cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014],
Conjugated Antibody
This product is also available conjugated. Format: E20-75087-Conjugate. Price: $485 / Size: 100μl.
E20-75087-FITC$485 / 100μlE20-75087-Biotin$485 / 100μlE20-75087-HRP$485 / 100μlE20-75087-AP$485 / 100μlE20-75087-AF350$485 / 100μlE20-75087-AF405M$485 / 100μlE20-75087-AF488$485 / 100μlE20-75087-AF514$485 / 100μlE20-75087-AF532$485 / 100μlE20-75087-AF555$485 / 100μlE20-75087-AF568$485 / 100μlE20-75087-AF405S$485 / 100μlE20-75087-AF405L$485 / 100μlE20-75087-AF546$485 / 100μlE20-75087-AF594$485 / 100μlE20-75087-AF610$485 / 100μlE20-75087-AF635$485 / 100μlE20-75087-AF647$485 / 100μlE20-75087-AF680$485 / 100μlE20-75087-AF700$485 / 100μlE20-75087-AF750$485 / 100μlE20-75087-AF790$485 / 100μlE20-75087-APC$485 / 100μlE20-75087-PE$485 / 100μlE20-75087-Cy3$485 / 100μlE20-75087-Cy5$485 / 100μlE20-75087-Cy5.5$485 / 100μlE20-75087-Cy7$485 / 100μlE20-75087-PE-Cy5$485 / 100μl
Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.
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