World supplier of antibodies, antigens and kits since 2007
Cell Signal Transduction Research Antibodies
UHRF1 Polyclonal Antibody
Polyclonal, Rabbit
$225/ 100ul
QuantityTotal: $225
Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.
Product Information
Molecular Weight89814Da
Protein NameE3 ubiquitin-protein ligase UHRF1
Human Gene Id29128
Human Swiss Prot NoQ96T88
Mouse Swiss Prot NoQ8VDF2
SpecificityUHRF1 Polyclonal Antibody detects endogenous levels of UHRF1 protein.
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration1 mg/ml
Storage Stability-20°C/1 year
Other NameUHRF1; ICBP90; NP95; RNF106; E3 ubiquitin-protein ligase UHRF1; Inverted CCAAT box-binding protein of 90 kDa; Nuclear protein 95; Nuclear zinc finger protein Np95; HuNp95; hNp95; RING finger protein 106;Transcription factor ICBP90; Ubiquitin-like PHD and RING finger domain-containing protein 1; hUHRF1; Ubiquitin-like-containing PHD and RING finger domains protein 1
Observed Band(KD)89
Functiondevelopmental stage:Expressed in fetal thymus, liver and kidney.,domain:The RING finger is required for ubiquitin ligase activity.,domain:The YDG domain mediates the interaction with histone H3.,function:Putative E3 ubiquitin-protein ligase. May participate in methylation-dependent transcriptional regulation. Binds to inverted 5'-CCAAT-3' box 2 in the TOP2A promoter, and activates TOP2A expression. Important for G1/S transition. May be involved in DNA repair and chromosomal stability.,induction:Up-regulated in proliferating cells, and down-regulated in quiescent cells. Down-regulated upon adriamycin-induced DNA damage, in a TP53/p53 and CDKN1A-dependent way. Induced by E2F1 transcription factor.,pathway:Protein modification; protein ubiquitination.,PTM:Phosphorylated on serine residues. Phosphorylation may enhance DNA-binding activity.,PTM:Ubiquitinated; which leads to proteasomal degrad
Subcellular LocationNucleus . Localizes to replication foci. Enriched in pericentric heterochromatin. Also localizes to euchromatic regions.
ExpressionExpressed in thymus, bone marrow, testis, lung and heart. Overexpressed in breast cancer.
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
ImmunogenSynthesized peptide derived from the Internal region of human UHRF1.
Gene NameUHRF1
DilutionWestern Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Backgroundubiquitin like with PHD and ring finger domains 1(UHRF1) Homo sapiens This gene encodes a member of a subfamily of RING-finger type E3 ubiquitin ligases. The protein binds to specific DNA sequences, and recruits a histone deacetylase to regulate gene expression. Its expression peaks at late G1 phase and continues during G2 and M phases of the cell cycle. It plays a major role in the G1/S transition by regulating topoisomerase IIalpha and retinoblastoma gene expression, and functions in the p53-dependent DNA damage checkpoint. It is regarded as a hub protein for the integration of epigenetic information. This gene is up-regulated in various cancers, and it is therefore considered to be a therapeutic target. Multiple transcript variants encoding different isoforms have been found for this gene. A related pseudogene exists on chromosome 12. [provided by RefSeq, Feb 2014],
Conjugated Antibody
This product is also available conjugated. Format: E20-75066-Conjugate. Price: $485 / Size: 100μl.
E20-75066-FITC$485 / 100μlE20-75066-Biotin$485 / 100μlE20-75066-HRP$485 / 100μlE20-75066-AP$485 / 100μlE20-75066-AF350$485 / 100μlE20-75066-AF405M$485 / 100μlE20-75066-AF488$485 / 100μlE20-75066-AF514$485 / 100μlE20-75066-AF532$485 / 100μlE20-75066-AF555$485 / 100μlE20-75066-AF568$485 / 100μlE20-75066-AF405S$485 / 100μlE20-75066-AF405L$485 / 100μlE20-75066-AF546$485 / 100μlE20-75066-AF594$485 / 100μlE20-75066-AF610$485 / 100μlE20-75066-AF635$485 / 100μlE20-75066-AF647$485 / 100μlE20-75066-AF680$485 / 100μlE20-75066-AF700$485 / 100μlE20-75066-AF750$485 / 100μlE20-75066-AF790$485 / 100μlE20-75066-APC$485 / 100μlE20-75066-PE$485 / 100μlE20-75066-Cy3$485 / 100μlE20-75066-Cy5$485 / 100μlE20-75066-Cy5.5$485 / 100μlE20-75066-Cy7$485 / 100μlE20-75066-PE-Cy5$485 / 100μl
Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.
Categories by research area
Categories by product type
- Antibodies
- Tag antibodies
- Internal control antibodies
- Pathological (Immunohistochemistry, IHC) antibodies
- Cell Signal Transduction Research Antibodies
- Secondary Antibody
- Antibody Sampler Kits
- Recombinant Proteins
- Fluorescence Reagents
- Signal Inhibitors and Activators
- Transfection Reagent
- Cell biology kits