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Cell Signal Transduction Research Antibodies
Plk1 Polyclonal Antibody
Polyclonal, Rabbit
$225/ 100ul
QuantityTotal: $225
Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.
Product Information
Molecular Weight68255Da
Protein NameSerine/threonine-protein kinase PLK1
Human Gene Id5347
Human Swiss Prot NoP53350
Mouse Gene Id18817
Mouse Swiss Prot NoQ07832
Rat Gene Id25515
Rat Swiss Prot NoQ62673
SpecificityPlk1 Polyclonal Antibody detects endogenous levels of Plk1 protein.
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration1 mg/ml
Storage Stability-20°C/1 year
Other NamePLK1; PLK; Serine/threonine-protein kinase PLK1; Polo-like kinase 1; PLK-1; Serine/threonine-protein kinase 13; STPK13
Observed Band(KD)68
Functioncatalytic activity:ATP + a protein = ADP + a phosphoprotein.,developmental stage:Accumulates to a maximum during the G2 and M phases, declines to a nearly undetectable level following mitosis and throughout G1 phase, and then begins to accumulate again during S phase.,enzyme regulation:Activated by serine and threonine phosphorylation.,function:Serine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of APC/C inhibitors, and the regulation of mitotic exit and cytokinesis.,induction:By growth-stimulating agents.,PTM:Autophosphorylation and phosphorylation of Ser-137 are not significant events during activation of PLK1 in M phase.,PTM:Catalytic activity is enhanced by phosphorylation of Thr-210 and/or S
Subcellular LocationNucleus. Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Cytoplasm, cytoskeleton, spindle . Midbody . localization at the centrosome starts at the G1/S transition (PubMed:24018379). During early stages of mitosis, the phosphorylated form is detected on centrosomes and kinetochores. Localizes to the outer kinetochore. Presence of SGO1 and interaction with the phosphorylated form of BUB1 is required for the kinetochore localization. Localizes onto the central spindle by phosphorylating and docking at midzone proteins KIF20A/MKLP2 and PRC1. Colocalizes with FRY to separating centrosomes and spindle poles from prophase to metaphase in mitosis, but not in other stages of the cell cycle. Localization to the centrosome is required for S ph
ExpressionPlacenta and colon.
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
ImmunogenSynthesized peptide derived from Plk1 . at AA range: 80-160
Gene NamePLK1
DilutionWestern Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Backgroundpolo like kinase 1(PLK1) Homo sapiens The Ser/Thr protein kinase encoded by this gene belongs to the CDC5/Polo subfamily. It is highly expressed during mitosis and elevated levels are found in many different types of cancer. Depletion of this protein in cancer cells dramatically inhibited cell proliferation and induced apoptosis; hence, it is a target for cancer therapy. [provided by RefSeq, Sep 2015],
Conjugated Antibody
This product is also available conjugated. Format: E20-75054-Conjugate. Price: $485 / Size: 100μl.
E20-75054-FITC$485 / 100μlE20-75054-Biotin$485 / 100μlE20-75054-HRP$485 / 100μlE20-75054-AP$485 / 100μlE20-75054-AF350$485 / 100μlE20-75054-AF405M$485 / 100μlE20-75054-AF488$485 / 100μlE20-75054-AF514$485 / 100μlE20-75054-AF532$485 / 100μlE20-75054-AF555$485 / 100μlE20-75054-AF568$485 / 100μlE20-75054-AF405S$485 / 100μlE20-75054-AF405L$485 / 100μlE20-75054-AF546$485 / 100μlE20-75054-AF594$485 / 100μlE20-75054-AF610$485 / 100μlE20-75054-AF635$485 / 100μlE20-75054-AF647$485 / 100μlE20-75054-AF680$485 / 100μlE20-75054-AF700$485 / 100μlE20-75054-AF750$485 / 100μlE20-75054-AF790$485 / 100μlE20-75054-APC$485 / 100μlE20-75054-PE$485 / 100μlE20-75054-Cy3$485 / 100μlE20-75054-Cy5$485 / 100μlE20-75054-Cy5.5$485 / 100μlE20-75054-Cy7$485 / 100μlE20-75054-PE-Cy5$485 / 100μl
Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.
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