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Cell Signal Transduction Research Antibodies
GAPDH Polyclonal Antibody
Polyclonal, Rabbit
$225/ 100ul
QuantityTotal: $225
Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
ImmunogenSynthesized peptide derived from the N-terminal region of human GAPDH.
Gene NameGAPDH
DilutionWestern Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.
Product Information
Protein NameGlyceraldehyde-3-phosphate dehydrogenase
Human Gene Id2597
Human Swiss Prot NoP04406
Mouse Gene Id1.00042e+008
Mouse Swiss Prot NoP16858
Rat Gene Id24383
Rat Swiss Prot NoP04797
SpecificityGAPDH Polyclonal Antibody detects endogenous levels of GAPDH protein.
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.04% sodium azide.
Concentration1 mg/ml
Storage Stability-20°C/1 year
Other NameGAPDH; GAPD; CDABP0047; OK/SW-cl.12; Glyceraldehyde-3-phosphate dehydrogenase; GAPDH; Peptidyl-cysteine S-nitrosylase GAPDH
Observed Band(KD)36
Functioncatalytic activity:D-glyceraldehyde 3-phosphate + phosphate + NAD(+) = 3-phospho-D-glyceroyl phosphate + NADH.,function:Independent of its glycolytic activity it is also involved in membrane trafficking in the early secretory pathway.,online information:Glyceraldehyde 3-phosphate dehydrogenase entry,pathway:Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1.,pathway:Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.,PTM:Reversible S-nitrosylation of Cys-152 inhibits enzymatic activity and increases endogenous ADP-ribosylation, which inhibits the enzyme in a non-reversible manner. The latter modification is more likely to be a pathophysiological event associated with inhibition of gluconeogenesis.,sequence caution:Differs quite extensively.,similarity:Belongs to the glyceraldehyde-3-phosphate dehydrogenase fami
Subcellular LocationCytoplasm, cytosol . Nucleus . Cytoplasm, perinuclear region . Membrane . Cytoplasm, cytoskeleton . Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity). Postnuclear and Perinuclear regions (PubMed:12829261). .
ExpressionAstrocytoma,Brain,Cajal-Retzius cell,Colon adenocarcinoma,Epitheliu
Backgroundglyceraldehyde-3-phosphate dehydrogenase(GAPDH) Homo sapiens This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferri
Conjugated Antibody
This product is also available conjugated. Format: E20-75052-Conjugate. Price: $485 / Size: 100μl.
E20-75052-FITC$485 / 100μlE20-75052-Biotin$485 / 100μlE20-75052-HRP$485 / 100μlE20-75052-AP$485 / 100μlE20-75052-AF350$485 / 100μlE20-75052-AF405M$485 / 100μlE20-75052-AF488$485 / 100μlE20-75052-AF514$485 / 100μlE20-75052-AF532$485 / 100μlE20-75052-AF555$485 / 100μlE20-75052-AF568$485 / 100μlE20-75052-AF405S$485 / 100μlE20-75052-AF405L$485 / 100μlE20-75052-AF546$485 / 100μlE20-75052-AF594$485 / 100μlE20-75052-AF610$485 / 100μlE20-75052-AF635$485 / 100μlE20-75052-AF647$485 / 100μlE20-75052-AF680$485 / 100μlE20-75052-AF700$485 / 100μlE20-75052-AF750$485 / 100μlE20-75052-AF790$485 / 100μlE20-75052-APC$485 / 100μlE20-75052-PE$485 / 100μlE20-75052-Cy3$485 / 100μlE20-75052-Cy5$485 / 100μlE20-75052-Cy5.5$485 / 100μlE20-75052-Cy7$485 / 100μlE20-75052-PE-Cy5$485 / 100μl
Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.
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