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Cell Signal Transduction Research Antibodies
Topo IIα Polyclonal Antibody
Polyclonal, Rabbit
$225/ 100ul
QuantityTotal: $225
Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.
Product Information
Molecular Weight174385Da
Protein NameDNA topoisomerase 2-alpha
Human Gene Id7153
Human Swiss Prot NoP11388
Mouse Gene Id21973
Mouse Swiss Prot NoQ01320
SpecificityTopo IIα Polyclonal Antibody detects endogenous levels of Topo IIα protein.
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration1 mg/ml
Storage Stability-20°C/1 year
Other NameTOP2A; TOP2; DNA topoisomerase 2-alpha; DNA topoisomerase II, alpha isozyme
Observed Band(KD)174
Functioncatalytic activity:ATP-dependent breakage, passage and rejoining of double-stranded DNA.,enzyme regulation:Specifically inhibited by the intercalating agent amsacrine.,function:Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks.,miscellaneous:Eukaryotic topoisomerase I and II can relax both negative and positive supercoils, whereas prokaryotic enzymes relax only negative supercoils.,PTM:Phosphorylation has no effect on catalytic activity.,similarity:Belongs to the type II topoisomerase family.,subcellular location:Generally located in the nucleoplasm.,subunit:Homodimer. Interacts with COPS5.,
Subcellular LocationCytoplasm . Nucleus, nucleoplasm . Nucleus . Nucleus, nucleolus .
ExpressionExpressed in the tonsil, spleen, lymph node, thymus, skin, pancreas, testis, colon, kidney, liver, brain and lung (PubMed:9155056). Also found in high-grade lymphomas, squamous cell lung tumors and seminomas (PubMed:9155056).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
ImmunogenSynthesized peptide derived from Topo IIα . at AA range: 1160-1240
Gene NameTOP2A
DilutionWestern Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.
Backgroundtopoisomerase (DNA) II alpha(TOP2A) Homo sapiens This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromosome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also pla
Conjugated Antibody
This product is also available conjugated. Format: E20-75049-Conjugate. Price: $485 / Size: 100μl.
E20-75049-FITC$485 / 100μlE20-75049-Biotin$485 / 100μlE20-75049-HRP$485 / 100μlE20-75049-AP$485 / 100μlE20-75049-AF350$485 / 100μlE20-75049-AF405M$485 / 100μlE20-75049-AF488$485 / 100μlE20-75049-AF514$485 / 100μlE20-75049-AF532$485 / 100μlE20-75049-AF555$485 / 100μlE20-75049-AF568$485 / 100μlE20-75049-AF405S$485 / 100μlE20-75049-AF405L$485 / 100μlE20-75049-AF546$485 / 100μlE20-75049-AF594$485 / 100μlE20-75049-AF610$485 / 100μlE20-75049-AF635$485 / 100μlE20-75049-AF647$485 / 100μlE20-75049-AF680$485 / 100μlE20-75049-AF700$485 / 100μlE20-75049-AF750$485 / 100μlE20-75049-AF790$485 / 100μlE20-75049-APC$485 / 100μlE20-75049-PE$485 / 100μlE20-75049-Cy3$485 / 100μlE20-75049-Cy5$485 / 100μlE20-75049-Cy5.5$485 / 100μlE20-75049-Cy7$485 / 100μlE20-75049-PE-Cy5$485 / 100μl
Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.
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