SAP 155 Polyclonal Antibody

Name: SAP 155 Polyclonal Antibody
Brand: EnoGene Biotech Co. Ltd
SKU: E20-75038
Price: 225 USD
Availability: InStock

E20-75038Cite ID: EN0055171Datasheet COA MSDS

SourcePolyclonal, Rabbit

$225/ 100ul

Quantity

Total: $225

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

OverviewImages & Data (2)Documents

Product Information

Molecular Weight145830Da

Protein NameSplicing factor 3B subunit 1

Human Gene Id23451

Human Swiss Prot NoO75533

Mouse Swiss Prot NoQ99NB9

SpecificitySAP 155 Polyclonal Antibody detects endogenous levels of SAP 155 protein.

FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration1 mg/ml

Storage Stability-20°C/1 year

Other NameSF3B1; SAP155; Splicing factor 3B subunit 1; Pre-mRNA-splicing factor SF3b 155 kDa subunit; SF3b155; Spliceosome-associated protein 155; SAP 155

Observed Band(KD)145

Functionfunction:Subunit of the splicing factor SF3B required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex. Belongs also to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron.,PTM:Phosphorylated. Phosphorylation occurs concomitantly with the splicing catalytic steps. Phosphorylation on Thr-244, Thr-248 and Thr-313 by cyclin-dependent kinases promotes interaction with PPP1R8 during mitosis.,similarity:Belongs to the SF3B1 family.,similarity:Contains 11 HEAT repeats.,subcellular location:During mitosis, transiently dispersed from the nuclear speckles to the cytoplasm.,subunit:Identified

Subcellular LocationNucleus . Nucleus speckle. During mitosis, transiently dispersed from the nuclear speckles to the cytoplasm.

ExpressionBrain,Epithelium,Pooled,Testis,Uterus,

PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

ImmunogenThe antiserum was produced against synthesized peptide derived from human SAP 155. AA range:75-124

Gene NameSF3B1

DilutionWestern Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.

Backgroundsplicing factor 3b subunit 1(SF3B1) Homo sapiens This gene encodes subunit 1 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. The carboxy-terminal two-thirds of subunit 1 have 22 non-identical, tandem HEAT repeats that form rod-like, helical structures. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008],

📄Datasheet
Full product datasheet (PDF)✅Certificate of Analysis
Lot-specific COA (generated from product data)🧪MSDS
Material safety data sheet (generated)

Conjugated Antibody

This product is also available conjugated. Format: E20-75038-Conjugate. Price: $485 / Size: 100μl.

E20-75038-FITC$485 / 100μlE20-75038-Biotin$485 / 100μlE20-75038-HRP$485 / 100μlE20-75038-AP$485 / 100μlE20-75038-AF350$485 / 100μlE20-75038-AF405M$485 / 100μlE20-75038-AF488$485 / 100μlE20-75038-AF514$485 / 100μlE20-75038-AF532$485 / 100μlE20-75038-AF555$485 / 100μlE20-75038-AF568$485 / 100μlE20-75038-AF405S$485 / 100μlE20-75038-AF405L$485 / 100μlE20-75038-AF546$485 / 100μlE20-75038-AF594$485 / 100μlE20-75038-AF610$485 / 100μlE20-75038-AF635$485 / 100μlE20-75038-AF647$485 / 100μlE20-75038-AF680$485 / 100μlE20-75038-AF700$485 / 100μlE20-75038-AF750$485 / 100μlE20-75038-AF790$485 / 100μlE20-75038-APC$485 / 100μlE20-75038-PE$485 / 100μlE20-75038-Cy3$485 / 100μlE20-75038-Cy5$485 / 100μlE20-75038-Cy5.5$485 / 100μlE20-75038-Cy7$485 / 100μlE20-75038-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.