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Cell Signal Transduction Research Antibodies
NF90 Polyclonal Antibody
Polyclonal, Rabbit
$225/ 100ul
QuantityTotal: $225
Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.
Product Information
Molecular Weight95338Da
Protein NameInterleukin enhancer-binding factor 3
Human Gene Id3609
Human Swiss Prot NoQ12906
Mouse Gene Id16201
Mouse Swiss Prot NoQ9Z1X4
Rat Gene Id84472
Rat Swiss Prot NoQ9JIL3
SpecificityNF90 Polyclonal Antibody detects endogenous levels of NF90 protein.
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration1 mg/ml
Storage Stability-20°C/1 year
Other NameILF3; DRBF; MPHOSPH4; NF90; Interleukin enhancer-binding factor 3; Double-stranded RNA-binding protein 76; DRBP76; M-phase phosphoprotein 4; MPP4;Nuclear factor associated with dsRNA; NFAR; Nuclear factor of activated T-cells 90 kDa; NF-AT-90; Translational control protein 80; TCP80
Observed Band(KD)95
Functionfunction:May facilitate double-stranded RNA-regulated gene expression at the level of post-transcription. Can act as a translation inhibitory protein which binds to coding sequences of acid beta-glucosidase (GCase) and other mRNAs and functions at the initiation phase of GCase mRNA translation, probably by inhibiting its binding to polysomes. Can regulate protein arginine N-methyltransferase 1 activity. May regulate transcription of the IL2 gene during T-cell activation. Can promote the formation of stable DNA-dependent protein kinase holoenzyme complexes on DNA.,PTM:Arg-609 is dimethylated, probably to asymmetric dimethylarginine.,PTM:Methylated by protein arginine N-methyltransferase 1.,PTM:Phosphorylated by RNA-dependent protein kinase (EIF2AK2).,sequence caution:Contaminating sequence. Potential poly-A sequence.,sequence caution:Sequencing errors.,similarity:Contains 1 DZF domain.,si
Subcellular LocationNucleus, nucleolus . Cytoplasm . Nucleus . Localizes in the cytoplasm in response to viral infection. The unphosphorylated form is retained in the nucleus by ILF2. Phosphorylation at Thr-188 and Thr-315 causes the dissociation of ILF2 from the ILF2-ILF3 complex resulting in a cytoplasmic sequestration of ILF3. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. .
ExpressionUbiquitous.
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
ImmunogenThe antiserum was produced against synthesized peptide derived from human NF90. AA range:302-351
Gene NameILF3
DilutionWestern Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Backgroundinterleukin enhancer binding factor 3(ILF3) Homo sapiens This gene encodes a double-stranded RNA (dsRNA) binding protein that complexes with other proteins, dsRNAs, small noncoding RNAs, and mRNAs to regulate gene expression and stabilize mRNAs. This protein (NF90, ILF3) forms a heterodimer with a 45 kDa transcription factor (NF45, ILF2) required for T-cell expression of interleukin 2. This complex has been shown to affect the redistribution of nuclear mRNA to the cytoplasm. Knockdown of NF45 or NF90 protein retards cell growth, possibly by inhibition of mRNA stabilization. In contrast, an isoform (NF110) of this gene that is predominantly restricted to the nucleus has only minor effects on cell growth when its levels are reduced. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Dec 2014],
Conjugated Antibody
This product is also available conjugated. Format: E20-75036-Conjugate. Price: $485 / Size: 100μl.
E20-75036-FITC$485 / 100μlE20-75036-Biotin$485 / 100μlE20-75036-HRP$485 / 100μlE20-75036-AP$485 / 100μlE20-75036-AF350$485 / 100μlE20-75036-AF405M$485 / 100μlE20-75036-AF488$485 / 100μlE20-75036-AF514$485 / 100μlE20-75036-AF532$485 / 100μlE20-75036-AF555$485 / 100μlE20-75036-AF568$485 / 100μlE20-75036-AF405S$485 / 100μlE20-75036-AF405L$485 / 100μlE20-75036-AF546$485 / 100μlE20-75036-AF594$485 / 100μlE20-75036-AF610$485 / 100μlE20-75036-AF635$485 / 100μlE20-75036-AF647$485 / 100μlE20-75036-AF680$485 / 100μlE20-75036-AF700$485 / 100μlE20-75036-AF750$485 / 100μlE20-75036-AF790$485 / 100μlE20-75036-APC$485 / 100μlE20-75036-PE$485 / 100μlE20-75036-Cy3$485 / 100μlE20-75036-Cy5$485 / 100μlE20-75036-Cy5.5$485 / 100μlE20-75036-Cy7$485 / 100μlE20-75036-PE-Cy5$485 / 100μl
Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.
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