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Cell Signal Transduction Research Antibodies

V-ATPase D1 Polyclonal Antibody

E20-75026Cite ID: EN0055159DatasheetCOAMSDS
Polyclonal, Rabbit
$225/ 100ul
Quantity
Total: $225

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

Product Information

Molecular Weight40329Da
Protein NameV-type proton ATPase subunit d 1
Human Gene Id9114
Human Swiss Prot NoP61421
Mouse Gene Id11972
Mouse Swiss Prot NoP51863
SpecificityV-ATPase D1 Polyclonal Antibody detects endogenous levels of V-ATPase D1 protein.
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration1 mg/ml
Storage Stability-20°C/1 year
Other NameATP6V0D1; ATP6D; VPATPD; V-type proton ATPase subunit d 1; V-ATPase subunit d 1; 32 kDa accessory protein; V-ATPase 40 kDa accessory protein; V-ATPase AC39 subunit; p39; Vacuolar proton pump subunit d 1
Observed Band(KD)40
Functionfunction:Subunit of the integral membrane V0 complex of vacuolar ATPase. Vacuolar ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells, thus providing most of the energy required for transport processes in the vacuolar system. May play a role in coupling of proton transport and ATP hydrolysis.,similarity:Belongs to the V-ATPase V0D/AC39 subunit family.,subunit:V-ATPase is an heteromultimeric enzyme composed of a peripheral catalytic V1 complex (components A to H) attached to an integral membrane V0 proton pore complex (components: a, c, c', c'' and d).,tissue specificity:Ubiquitous.,
Subcellular LocationMembrane ; Peripheral membrane protein ; Cytoplasmic side . Lysosome membrane ; Peripheral membrane protein . Cytoplasmic vesicle, clathrin-coated vesicle membrane ; Peripheral membrane protein . Localizes to centrosome and the base of the cilium. .
ExpressionUbiquitous.
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
ImmunogenThe antiserum was produced against synthesized peptide derived from human V-ATPase D1. AA range:221-270
Gene NameATP6V0D1
DilutionWestern Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
BackgroundATPase H+ transporting V0 subunit d1(ATP6V0D1) Homo sapiens This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c'', and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is known as the D subunit and is found ubiquitously. [pro

Conjugated Antibody

This product is also available conjugated. Format: E20-75026-Conjugate. Price: $485 / Size: 100μl.

E20-75026-FITC$485 / 100μlE20-75026-Biotin$485 / 100μlE20-75026-HRP$485 / 100μlE20-75026-AP$485 / 100μlE20-75026-AF350$485 / 100μlE20-75026-AF405M$485 / 100μlE20-75026-AF488$485 / 100μlE20-75026-AF514$485 / 100μlE20-75026-AF532$485 / 100μlE20-75026-AF555$485 / 100μlE20-75026-AF568$485 / 100μlE20-75026-AF405S$485 / 100μlE20-75026-AF405L$485 / 100μlE20-75026-AF546$485 / 100μlE20-75026-AF594$485 / 100μlE20-75026-AF610$485 / 100μlE20-75026-AF635$485 / 100μlE20-75026-AF647$485 / 100μlE20-75026-AF680$485 / 100μlE20-75026-AF700$485 / 100μlE20-75026-AF750$485 / 100μlE20-75026-AF790$485 / 100μlE20-75026-APC$485 / 100μlE20-75026-PE$485 / 100μlE20-75026-Cy3$485 / 100μlE20-75026-Cy5$485 / 100μlE20-75026-Cy5.5$485 / 100μlE20-75026-Cy7$485 / 100μlE20-75026-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.