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Cell Signal Transduction Research Antibodies
CAF-1 p150 Polyclonal Antibody
Human;Mouse
WB;ELISA
Polyclonal, Rabbit,IgG
$225/ 100ul
QuantityTotal: $225
Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.
Product Information
Molecular Weight105223Da
Protein NameChromatin assembly factor 1 subunit A
Human Gene Id10036
Human Swiss Prot NoQ13111
Mouse Gene Id27221
Mouse Swiss Prot NoQ9QWF0
SpecificityCAF-1 p150 Polyclonal Antibody detects endogenous levels of CAF-1 p150 protein.
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration1 mg/ml
Storage Stability-20°C/1 year
Other NameCHAF1A; CAF; CAF1P150; Chromatin assembly factor 1 subunit A; CAF-1 subunit A; Chromatin assembly factor I p150 subunit; CAF-I 150 kDa subunit; CAF-I p150; hp150
Observed Band(KD)150
Functionalternative products:Experimental confirmation may be lacking for some isoforms,developmental stage:Active complex is found in G1, S and G2 phases.,domain:Contains one Pro-Xaa-Val-Xaa-Leu (PxVxL) motif, which is required for interaction with chromoshadow domains. This motif requires additional residues -7, -6, +4 and +5 of the central Val which contact the chromoshadow domain.,function:Core component of the CAF-1 complex, a complex thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. CHAF1A binds to histones H3 and H4. It may play a role in heterochromatin maintenanc
Subcellular LocationNucleus . DNA replication foci.
ExpressionBrain,Cervix adenocarcinoma,Epithelium,Ovary,
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
ImmunogenSynthesized peptide derived from CAF-1 p150 . at AA range: 300-380
Gene NameCHAF1A
DilutionWestern Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
BackgroundChromatin assembly factor I (CAF1) is a nuclear complex consisting of p50, p60 (CHAF1B; MIM 601245), and p150 (CHAF1A) subunits that assembles histone octamers onto replicating DNA in vitro (Kaufman et al., 1995 [PubMed 7600578]).[supplied by OMIM, Mar 2008],
Conjugated Antibody
This product is also available conjugated. Format: E20-75017-Conjugate. Price: $485 / Size: 100μl.
E20-75017-FITC$485 / 100μlE20-75017-Biotin$485 / 100μlE20-75017-HRP$485 / 100μlE20-75017-AP$485 / 100μlE20-75017-AF350$485 / 100μlE20-75017-AF405M$485 / 100μlE20-75017-AF488$485 / 100μlE20-75017-AF514$485 / 100μlE20-75017-AF532$485 / 100μlE20-75017-AF555$485 / 100μlE20-75017-AF568$485 / 100μlE20-75017-AF405S$485 / 100μlE20-75017-AF405L$485 / 100μlE20-75017-AF546$485 / 100μlE20-75017-AF594$485 / 100μlE20-75017-AF610$485 / 100μlE20-75017-AF635$485 / 100μlE20-75017-AF647$485 / 100μlE20-75017-AF680$485 / 100μlE20-75017-AF700$485 / 100μlE20-75017-AF750$485 / 100μlE20-75017-AF790$485 / 100μlE20-75017-APC$485 / 100μlE20-75017-PE$485 / 100μlE20-75017-Cy3$485 / 100μlE20-75017-Cy5$485 / 100μlE20-75017-Cy5.5$485 / 100μlE20-75017-Cy7$485 / 100μlE20-75017-PE-Cy5$485 / 100μl
Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.
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