XRCC4 Polyclonal Antibody

Name: XRCC4 Polyclonal Antibody
Brand: EnoGene Biotech Co. Ltd
SKU: E20-74921
Price: 225 USD
Availability: InStock

E20-74921Cite ID: EN0055054Datasheet COA MSDS

SourcePolyclonal, Rabbit

$225/ 100ul

Quantity

Total: $225

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

OverviewImages & Data (1)Documents

Product Information

Molecular Weight38287Da

Protein NameDNA repair protein XRCC4

Human Gene Id7518

Human Swiss Prot NoQ13426

Mouse Swiss Prot NoQ924T3

SpecificityXRCC4 Polyclonal Antibody detects endogenous levels of XRCC4 protein.

FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration1 mg/ml

Storage Stability-20°C/1 year

Other NameXRCC4; DNA repair protein XRCC4; X-ray repair cross-complementing protein 4

Observed Band(KD)40

Functionfunction:Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. Binds to DNA and to DNA ligase IV (LIG4). The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends.,PTM:Monoubiquitinated.,PTM:Phosphorylated by PRKDC. The phosphorylation seems not to be necessary for binding to DNA. Phosphorylation by CK2 promotes interaction with APTX.,PTM:Sumoylation at Lys-210 is required for nuclear localization and recombination efficiency. Has no effect on ubiquitination.,similarity:Belongs to the XRCC4 family.,subunit:Homodimer and homotetramer in solution. The homodimer associates with LIG4, and the LIG4-XRCC4 complex associates in a DNA-dep

Subcellular LocationNucleus . Chromosome . Localizes to site of double-strand breaks. .; [Protein XRCC4, C-terminus]: Cytoplasm . Translocates from the nucleus to the cytoplasm following cleavage by caspase-3 (CASP3). .

ExpressionWidely expressed.

PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

ImmunogenThe antiserum was produced against synthesized peptide derived from human XRCC4. AA range:261-310

Gene NameXRCC4

DilutionWestern Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.

BackgroundX-ray repair cross complementing 4(XRCC4) Homo sapiens The protein encoded by this gene functions together with DNA ligase IV and the DNA-dependent protein kinase in the repair of DNA double-strand breaks. This protein plays a role in both non-homologous end joining and the completion of V(D)J recombination. Mutations in this gene can cause short stature, microcephaly, and endocrine dysfunction (SSMED). Alternative splicing generates several transcript variants. [provided by RefSeq, Dec 2015],

📄Datasheet
Full product datasheet (PDF)✅Certificate of Analysis
Lot-specific COA (generated from product data)🧪MSDS
Material safety data sheet (generated)

Conjugated Antibody

This product is also available conjugated. Format: E20-74921-Conjugate. Price: $485 / Size: 100μl.

E20-74921-FITC$485 / 100μlE20-74921-Biotin$485 / 100μlE20-74921-HRP$485 / 100μlE20-74921-AP$485 / 100μlE20-74921-AF350$485 / 100μlE20-74921-AF405M$485 / 100μlE20-74921-AF488$485 / 100μlE20-74921-AF514$485 / 100μlE20-74921-AF532$485 / 100μlE20-74921-AF555$485 / 100μlE20-74921-AF568$485 / 100μlE20-74921-AF405S$485 / 100μlE20-74921-AF405L$485 / 100μlE20-74921-AF546$485 / 100μlE20-74921-AF594$485 / 100μlE20-74921-AF610$485 / 100μlE20-74921-AF635$485 / 100μlE20-74921-AF647$485 / 100μlE20-74921-AF680$485 / 100μlE20-74921-AF700$485 / 100μlE20-74921-AF750$485 / 100μlE20-74921-AF790$485 / 100μlE20-74921-APC$485 / 100μlE20-74921-PE$485 / 100μlE20-74921-Cy3$485 / 100μlE20-74921-Cy5$485 / 100μlE20-74921-Cy5.5$485 / 100μlE20-74921-Cy7$485 / 100μlE20-74921-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.