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Cell Signal Transduction Research Antibodies
WTAP Polyclonal Antibody
Polyclonal, Rabbit
$225/ 100ul
QuantityTotal: $225
Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.
Product Information
Molecular Weight44244Da
Protein NamePre-mRNA-splicing regulator WTAP
Human Gene Id9589
Human Swiss Prot NoQ15007
Mouse Gene Id60532
Mouse Swiss Prot NoQ9ER69
SpecificityWTAP Polyclonal Antibody detects endogenous levels of WTAP protein.
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration1 mg/ml
Storage Stability-20°C/1 year
Other NameWTAP; KIAA0105; Pre-mRNA-splicing regulator WTAP; Female-lethal(2)D homolog; hFL(2)D; WT1-associated protein; Wilms tumor 1-associating protein
Observed Band(KD)44
Functionfunction:Regulates G2/M cell-cycle transition by binding to the 3' UTR of CCNA2, which enhances its stability. Impairs WT1 DNA-binding ability and inhibits expression of WT1 target genes. May be involved in mRNA splicing regulation.,induction:In smooth muscle cells, up-regulated after serum withdrawal, when cells become mature and non proliferative.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the fl(2)d family.,subunit:Interacts with WT1.,tissue specificity:Ubiquitously expressed.,
Subcellular LocationNucleus speckle . Nucleus, nucleoplasm . Cytoplasm . Mainly nuclear with some fraction located in the cytoplasm. ZC3H13 is required to anchor component of the MACOM subcomplex, such as VIRMA, in the nucleus. .
ExpressionUbiquitously expressed.
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
ImmunogenThe antiserum was produced against synthesized peptide derived from human WTAP. AA range:321-370
Gene NameWTAP
DilutionWestern Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.
BackgroundWilms tumor 1 associated protein(WTAP) Homo sapiens The Wilms tumor suppressor gene WT1 appears to play a role in both transcriptional and posttranscriptional regulation of certain cellular genes. This gene encodes a WT1-associating protein, which is a ubiquitously expressed nuclear protein. Like WT1 protein, this protein is localized throughout the nucleoplasm as well as in speckles and partially colocalizes with splicing factors. Alternative splicing of this gene results in several transcript variants encoding three different isoforms. [provided by RefSeq, Jul 2012],
Conjugated Antibody
This product is also available conjugated. Format: E20-74908-Conjugate. Price: $485 / Size: 100μl.
E20-74908-FITC$485 / 100μlE20-74908-Biotin$485 / 100μlE20-74908-HRP$485 / 100μlE20-74908-AP$485 / 100μlE20-74908-AF350$485 / 100μlE20-74908-AF405M$485 / 100μlE20-74908-AF488$485 / 100μlE20-74908-AF514$485 / 100μlE20-74908-AF532$485 / 100μlE20-74908-AF555$485 / 100μlE20-74908-AF568$485 / 100μlE20-74908-AF405S$485 / 100μlE20-74908-AF405L$485 / 100μlE20-74908-AF546$485 / 100μlE20-74908-AF594$485 / 100μlE20-74908-AF610$485 / 100μlE20-74908-AF635$485 / 100μlE20-74908-AF647$485 / 100μlE20-74908-AF680$485 / 100μlE20-74908-AF700$485 / 100μlE20-74908-AF750$485 / 100μlE20-74908-AF790$485 / 100μlE20-74908-APC$485 / 100μlE20-74908-PE$485 / 100μlE20-74908-Cy3$485 / 100μlE20-74908-Cy5$485 / 100μlE20-74908-Cy5.5$485 / 100μlE20-74908-Cy7$485 / 100μlE20-74908-PE-Cy5$485 / 100μl
Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.
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