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Cell Signal Transduction Research Antibodies
WNK1 Polyclonal Antibody
Polyclonal, Rabbit
$225/ 100ul
QuantityTotal: $225
Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.
Product Information
Molecular Weight250756Da
Protein NameSerine/threonine-protein kinase WNK1
Human Gene Id65125
Human Swiss Prot NoQ9H4A3
Mouse Gene Id232341
Mouse Swiss Prot NoP83741
Rat Gene Id116477
Rat Swiss Prot NoQ9JIH7
SpecificityWNK1 Polyclonal Antibody detects endogenous levels of WNK1 protein.
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration1 mg/ml
Storage Stability-20°C/1 year
Other NameWNK1; HSN2; KDP; KIAA0344; PRKWNK1; Serine/threonine-protein kinase WNK1; Erythrocyte 65 kDa protein; p65; Kinase deficient protein; Protein kinase lysine-deficient 1; Protein kinase with no lysine 1; hWNK1
Observed Band(KD)230
Functioncatalytic activity:ATP + a protein = ADP + a phosphoprotein.,caution:Cys-250 is present instead of the conserved Lys which is expected to be an active site residue. Lys-233 appears to fulfill the required catalytic function.,caution:PubMed:2507249 describes a peptide sequence containing a GlcNAc glycosylated Ser in position 164 while it is an Arg residue according to others.,cofactor:Magnesium.,disease:Defects in WNK1 are a cause of pseudohypoaldosteronism type II (PHAII) [MIM:145260]. PHAII is an autosomal dominant disease characterized by severe hypertension, hyperkalemia, and sensitivity to thiazide diuretics which may result from a chloride shunt in the renal distal nephron.,enzyme regulation:By hypertonicity. Activation requires autophosphorylation of Ser-382. Phosphorylation of Ser-378 also promotes increased activity.,function:Controls sodium and chloride ion transport by inhibiti
Subcellular LocationCytoplasm .
ExpressionWidely expressed, with highest levels observed in the testis, heart, kidney and skeletal muscle. Isoform 3 is kidney-specific and specifically expressed in the distal convoluted tubule (DCT) and connecting tubule (CNT) of the nephron.
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
ImmunogenThe antiserum was produced against synthesized peptide derived from human WNK1. AA range:24-73
Gene NameWNK1
DilutionWestern Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.
BackgroundWNK lysine deficient protein kinase 1(WNK1) Homo sapiens This gene encodes a member of the WNK subfamily of serine/threonine protein kinases. The encoded protein may be a key regulator of blood pressure by controlling the transport of sodium and chloride ions. Mutations in this gene have been associated with pseudohypoaldosteronism type II and hereditary sensory neuropathy type II. Alternatively spliced transcript variants encoding different isoforms have been described but the full-length nature of all of them has yet to be determined.[provided by RefSeq, May 2010],
Conjugated Antibody
This product is also available conjugated. Format: E20-74906-Conjugate. Price: $485 / Size: 100μl.
E20-74906-FITC$485 / 100μlE20-74906-Biotin$485 / 100μlE20-74906-HRP$485 / 100μlE20-74906-AP$485 / 100μlE20-74906-AF350$485 / 100μlE20-74906-AF405M$485 / 100μlE20-74906-AF488$485 / 100μlE20-74906-AF514$485 / 100μlE20-74906-AF532$485 / 100μlE20-74906-AF555$485 / 100μlE20-74906-AF568$485 / 100μlE20-74906-AF405S$485 / 100μlE20-74906-AF405L$485 / 100μlE20-74906-AF546$485 / 100μlE20-74906-AF594$485 / 100μlE20-74906-AF610$485 / 100μlE20-74906-AF635$485 / 100μlE20-74906-AF647$485 / 100μlE20-74906-AF680$485 / 100μlE20-74906-AF700$485 / 100μlE20-74906-AF750$485 / 100μlE20-74906-AF790$485 / 100μlE20-74906-APC$485 / 100μlE20-74906-PE$485 / 100μlE20-74906-Cy3$485 / 100μlE20-74906-Cy5$485 / 100μlE20-74906-Cy5.5$485 / 100μlE20-74906-Cy7$485 / 100μlE20-74906-PE-Cy5$485 / 100μl
Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.
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