V-ATPase B1 Polyclonal Antibody

Name: V-ATPase B1 Polyclonal Antibody
Brand: EnoGene Biotech Co. Ltd
SKU: E20-74858
Price: 225 USD
Availability: InStock

E20-74858Cite ID: EN0054991Datasheet COA MSDS

SourcePolyclonal, Rabbit

$225/ 100ul

Quantity

Total: $225

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

OverviewImages & Data (1)Documents

Product Information

Molecular Weight56980Da

Protein NameV-type proton ATPase subunit B kidney isoform

Human Gene Id525

Human Swiss Prot NoP15313

SpecificityV-ATPase B1 Polyclonal Antibody detects endogenous levels of V-ATPase B1 protein.

FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration1 mg/ml

Storage Stability-20°C/1 year

Other NameATP6V1B1; ATP6B1; VATB; VPP3; V-type proton ATPase subunit B; kidney isoform; V-ATPase subunit B 1; Endomembrane proton pump 58 kDa subunit; Vacuolar proton pump subunit B 1

Observed Band(KD)60

Functiondisease:Defects in ATP6V1B1 are the cause of distal renal tubular acidosis with deafness (dRTA) [MIM:267300]. Inheritance is autosomal recessive. Patients with recessive dRTA are severely affected, presenting with either acute illness or growth failure at a young age, and bilateral sensorineural deafness. Other features include low serum K(+) due to renal potassium wasting, and elevated urinary calcium. If untreated, this acidosis may result in dissolution of bone, leading to osteomalacia and rickets. Renal deposition of calcium salts (nephrocalcinosis) and renal stone formation commonly occur.,domain:The PDZ-binding motif mediates interactions with SLC9A3R1 and SCL4A7.,function:Non-catalytic subunit of the peripheral V1 complex of vacuolar ATPase. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells.,similarity:Belongs to the ATPase alpha/be

Subcellular LocationApical cell membrane . Basolateral cell membrane .

ExpressionKidney; localizes to early distal nephron, encompassing thick ascending limbs and distal convoluted tubules (at protein level) (PubMed:29993276, PubMed:16769747). Expressed in the cochlea and endolymphatic sac (PubMed:9916796).

PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

ImmunogenThe antiserum was produced against synthesized peptide derived from human ATP6V1B1. AA range:381-430

Gene NameATP6V1B1

DilutionWestern Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.

BackgroundATPase H+ transporting V1 subunit B1(ATP6V1B1) Homo sapiens This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c'', and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is one of two V1 domain B subunit isoforms and is found i

📄Datasheet
Full product datasheet (PDF)✅Certificate of Analysis
Lot-specific COA (generated from product data)🧪MSDS
Material safety data sheet (generated)

Conjugated Antibody

This product is also available conjugated. Format: E20-74858-Conjugate. Price: $485 / Size: 100μl.

E20-74858-FITC$485 / 100μlE20-74858-Biotin$485 / 100μlE20-74858-HRP$485 / 100μlE20-74858-AP$485 / 100μlE20-74858-AF350$485 / 100μlE20-74858-AF405M$485 / 100μlE20-74858-AF488$485 / 100μlE20-74858-AF514$485 / 100μlE20-74858-AF532$485 / 100μlE20-74858-AF555$485 / 100μlE20-74858-AF568$485 / 100μlE20-74858-AF405S$485 / 100μlE20-74858-AF405L$485 / 100μlE20-74858-AF546$485 / 100μlE20-74858-AF594$485 / 100μlE20-74858-AF610$485 / 100μlE20-74858-AF635$485 / 100μlE20-74858-AF647$485 / 100μlE20-74858-AF680$485 / 100μlE20-74858-AF700$485 / 100μlE20-74858-AF750$485 / 100μlE20-74858-AF790$485 / 100μlE20-74858-APC$485 / 100μlE20-74858-PE$485 / 100μlE20-74858-Cy3$485 / 100μlE20-74858-Cy5$485 / 100μlE20-74858-Cy5.5$485 / 100μlE20-74858-Cy7$485 / 100μlE20-74858-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.