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Cell Signal Transduction Research Antibodies
VASP Polyclonal Antibody
Polyclonal, Rabbit
$225/ 100ul
QuantityTotal: $225
Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.
Product Information
Molecular Weight39699Da
Protein NameVasodilator-stimulated phosphoprotein
Human Gene Id7408
Human Swiss Prot NoP50552
Mouse Gene Id22323
Mouse Swiss Prot NoP70460
SpecificityVASP Polyclonal Antibody detects endogenous levels of VASP protein.
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration1 mg/ml
Storage Stability-20°C/1 year
Other NameVASP; Vasodilator-stimulated phosphoprotein; VASP
Observed Band(KD)46+50
Functiondomain:The EVH2 domain is comprised of 3 regions. Block A is a thymosin-like domain required for G-actin binding. The KLKR motif within this block is essential for the G-actin binding and for actin polymerization. Block B is required for F-actin binding and subcellular location, and Block C for tetramerization.,domain:The WH1 domain mediates interaction with XIRP1.,function:Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance and lamellipodial and filopodial dynamics in migrating cells. VASP promotes actin nucleation and increases the rate of actin polymerization in the presence of capping protein. Plays a role in actin-based activity of Listeria monocytogenes in platelets.,PTM:Major substrate for cAMP-dependent (PKA) and cGMP-dependent protein kinase (PKG) in platelets. The preferred
Subcellular LocationCytoplasm. Cytoplasm, cytoskeleton. Cell junction, focal adhesion. Cell junction, tight junction . Cell projection, lamellipodium membrane. Cell projection, filopodium membrane. Targeted to stress fibers and focal adhesions through interaction with a number of proteins including MRL family members. Localizes to the plasma membrane in protruding lamellipodia and filopodial tips. Stimulation by thrombin or PMA, also translocates VASP to focal adhesions. Localized along the sides of actin filaments throughout the peripheral cytoplasm under basal conditions. In pre-apoptotic cells, colocalizes with MEFV in large specks (pyroptosomes).
ExpressionHighly expressed in platelets.
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
ImmunogenThe antiserum was produced against synthesized peptide derived from human VASP. AA range:206-255
Gene NameVASP
DilutionWestern Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
Backgroundvasodilator-stimulated phosphoprotein(VASP) Homo sapiens Vasodilator-stimulated phosphoprotein (VASP) is a member of the Ena-VASP protein family. Ena-VASP family members contain an EHV1 N-terminal domain that binds proteins containing E/DFPPPPXD/E motifs and targets Ena-VASP proteins to focal adhesions. In the mid-region of the protein, family members have a proline-rich domain that binds SH3 and WW domain-containing proteins. Their C-terminal EVH2 domain mediates tetramerization and binds both G and F actin. VASP is associated with filamentous actin formation and likely plays a widespread role in cell adhesion and motility. VASP may also be involved in the intracellular signaling pathways that regulate integrin-extracellular matrix interactions. VASP is regulated by the cyclic nucleotide-dependent kinases PKA and PKG. [provided by RefSeq, Jul 2008],
Conjugated Antibody
This product is also available conjugated. Format: E20-74854-Conjugate. Price: $485 / Size: 100μl.
E20-74854-FITC$485 / 100μlE20-74854-Biotin$485 / 100μlE20-74854-HRP$485 / 100μlE20-74854-AP$485 / 100μlE20-74854-AF350$485 / 100μlE20-74854-AF405M$485 / 100μlE20-74854-AF488$485 / 100μlE20-74854-AF514$485 / 100μlE20-74854-AF532$485 / 100μlE20-74854-AF555$485 / 100μlE20-74854-AF568$485 / 100μlE20-74854-AF405S$485 / 100μlE20-74854-AF405L$485 / 100μlE20-74854-AF546$485 / 100μlE20-74854-AF594$485 / 100μlE20-74854-AF610$485 / 100μlE20-74854-AF635$485 / 100μlE20-74854-AF647$485 / 100μlE20-74854-AF680$485 / 100μlE20-74854-AF700$485 / 100μlE20-74854-AF750$485 / 100μlE20-74854-AF790$485 / 100μlE20-74854-APC$485 / 100μlE20-74854-PE$485 / 100μlE20-74854-Cy3$485 / 100μlE20-74854-Cy5$485 / 100μlE20-74854-Cy5.5$485 / 100μlE20-74854-Cy7$485 / 100μlE20-74854-PE-Cy5$485 / 100μl
Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.
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