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Cell Signal Transduction Research Antibodies
UCH-L1 Polyclonal Antibody
Polyclonal, Rabbit
$225/ 100ul
QuantityTotal: $225
Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.
Product Information
Molecular Weight24824Da
Protein NameUbiquitin carboxyl-terminal hydrolase isozyme L1
Human Gene Id7345
Human Swiss Prot NoP09936
Mouse Gene Id22223
Mouse Swiss Prot NoQ9R0P9
Rat Gene Id29545
Rat Swiss Prot NoQ00981
SpecificityUCH-L1 Polyclonal Antibody detects endogenous levels of UCH-L1 protein.
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration1 mg/ml
Storage Stability-20°C/1 year
Other NameUCHL1; Ubiquitin carboxyl-terminal hydrolase isozyme L1; UCH-L1; Neuron cytoplasmic protein 9.5; PGP 9.5; PGP9.5; Ubiquitin thioesterase L1
Observed Band(KD)28
Functioncatalytic activity:Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).,disease:Oxidation of Met-1, Met-6, Met-12, Met-124 and Met-179 to methionine sulfoxide, and oxidation of Cys-220 to cysteine sulfonic acid have been observed in brains from Alzheimer disease (AD) and Parkinson disease (PD) patients. In AD, UCHL1 was found to be associated with neurofibrillary tangles.,function:Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin
Subcellular LocationCytoplasm . Endoplasmic reticulum membrane ; Lipid-anchor . About 30% of total UCHL1 is associated with membranes in brain.
ExpressionFound in neuronal cell bodies and processes throughout the neocortex (at protein level). Expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. Weakly expressed in ovary. Down-regulated in brains from Parkinson disease and Alzheimer disease patients.
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
ImmunogenThe antiserum was produced against synthesized peptide derived from human PGP9.5. AA range:171-220
Gene NameUCHL1
DilutionWestern Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.
Backgroundubiquitin C-terminal hydrolase L1(UCHL1) Homo sapiens The protein encoded by this gene belongs to the peptidase C12 family. This enzyme is a thiol protease that hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. This gene is specifically expressed in the neurons and in cells of the diffuse neuroendocrine system. Mutations in this gene may be associated with Parkinson disease.[provided by RefSeq, Sep 2009],
Conjugated Antibody
This product is also available conjugated. Format: E20-74812-Conjugate. Price: $485 / Size: 100μl.
E20-74812-FITC$485 / 100μlE20-74812-Biotin$485 / 100μlE20-74812-HRP$485 / 100μlE20-74812-AP$485 / 100μlE20-74812-AF350$485 / 100μlE20-74812-AF405M$485 / 100μlE20-74812-AF488$485 / 100μlE20-74812-AF514$485 / 100μlE20-74812-AF532$485 / 100μlE20-74812-AF555$485 / 100μlE20-74812-AF568$485 / 100μlE20-74812-AF405S$485 / 100μlE20-74812-AF405L$485 / 100μlE20-74812-AF546$485 / 100μlE20-74812-AF594$485 / 100μlE20-74812-AF610$485 / 100μlE20-74812-AF635$485 / 100μlE20-74812-AF647$485 / 100μlE20-74812-AF680$485 / 100μlE20-74812-AF700$485 / 100μlE20-74812-AF750$485 / 100μlE20-74812-AF790$485 / 100μlE20-74812-APC$485 / 100μlE20-74812-PE$485 / 100μlE20-74812-Cy3$485 / 100μlE20-74812-Cy5$485 / 100μlE20-74812-Cy5.5$485 / 100μlE20-74812-Cy7$485 / 100μlE20-74812-PE-Cy5$485 / 100μl
Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.
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