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TULA Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-74782

  • Amount:

    100ul

  • Price:

    225$

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human PLCG1 around the phosphorylation site of Tyr1253. AA range:1221-1270

  • Source:

    Polyclonal, Rabbit

  • Gene Name:

    PLCG1

  • Dilution:

    Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.

  • Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using PLCG1 (Phospho-Tyr1253) Antibody

  • Background:

    phospholipase C gamma 1(PLCG1) Homo sapiens The protein encoded by this gene catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of receptor-mediated tyrosine kinase activators. For example, when activated by SRC, the encoded protein causes the Ras guanine nucleotide exchange factor RasGRP1 to translocate to the Golgi, where it activates Ras. Also, this protein has been shown to be a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008],

  • Protein Name:

    1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1

  • Human Gene Id:

    5335

  • Human Swiss Prot No:

    P19174

  • Mouse Gene Id:

    18803

  • Mouse Swiss Prot No:

    Q62077

  • Rat Gene Id:

    25738

  • Rat Swiss Prot No:

    P10686

  • Specificity:

    Phospho-PLC γ1 (Y1253) Polyclonal Antibody detects endogenous levels of PLC γ1 protein only when phosphorylated at Y1253.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    PLCG1; PLC1; 1-phosphatidylinositol 4; 5-bisphosphate phosphodiesterase gamma-1; PLC-148; Phosphoinositide phospholipase C-gamma-1; Phospholipase C-II; PLC-II; Phospholipase C-gamma-1; PLC-gamma-1

  • MolecularWeight(Da):

    148532

  • Observed Band(KD):

    150

  • Function:

    catalytic activity:1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H(2)O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.,cofactor:Calcium.,domain:The SH3 domain mediates interaction with CLNK (By similarity). The SH3 domain also mediates interaction with RALGPS1.,function:PLC-gamma is a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase.,PTM:The receptor-mediated activation of PLC-gamma-1 and PLC-gamma-2 involves their phosphorylation by tyrosine kinases in response to ligation of a variety of growth factor receptors and immune system receptors.,PTM:Ubiquitinated by CBLB in activated T-cells.,similarity:Contains 1 C2 domain.,similarity:Contains 1 EF-hand domain.,similarity:Contains 1 PH domain.,similarity:Contains 1 PI-PLC X-box domain.,similarity:Contains 1 PI-PLC Y-box domain.,similarity:Contains 1 SH3 domain.,simil

  • Subcellular Location:

    ruffle,intracellular,cytoplasm,cytosol,plasma membrane,cell-cell junction,COP9 signalosome,lamellipodium,cell projection,

  • Expression:

    Brain,Epithelium,Testis,Vein,

  • Western blot analysis of lysates from LOVO cells treated with and 293 cells treated with heat shock, using PLCG1 (Phospho-Tyr1253) Antibody. The lane on the right is blocked with the phospho peptide.

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Catalog Number

  • Amount:100ul
  • Price:225$

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TULA Polyclonal Antibody | EnoGene Biotech Co. Ltd