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TdT Polyclonal Antibody
Datasheet
Catalog Number:
E20-74594Amount:
100ulReactivity:
Human,Rat,MousePurification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenImmunogen:
The antiserum was produced against synthesized peptide derived from human DNTT. AA range:381-430Source:
Polyclonal, Rabbit
- Western Blot analysis of HepG2 cells using TdT Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA)
Gene Name:
DNTTDilution:
IHC-p: 100-300.Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applicationsProtein Name:
DNA nucleotidylexotransferaseHuman Gene Id:
1791Human Swiss Prot No:
P04053Specificity:
TdT Polyclonal Antibody detects endogenous levels of TdT proteinFormulation:
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azideConcentration:
1 mg/mlStorage Stability:
-20°C/1 yearOther Name:
DNTT; TDT; DNA nucleotidylexotransferase; Terminal addition enzyme; Terminal deoxynucleotidyltransferase; Terminal transferaseMolecularWeight:58437DaObserved Band(KD):
60Background:
DNA nucleotidylexotransferase(DNTT) Homo sapiens This gene is a member of the DNA polymerase type-X family and encodes a template-independent DNA polymerase that catalyzes the addition of deoxynucleotides to the 3'-hydroxyl terminus of oligonucleotide primers. In vivo, the encoded protein is expressed in a restricted population of normal and malignant pre-B and pre-T lymphocytes during early differentiation, where it generates antigen receptor diversity by synthesizing non-germ line elements (N-regions) at the junctions of rearranged Ig heavy chain and T cell receptor gene segments. Alternatively spliced transcript variants encoding different isoforms of this gene have been described. [provided by RefSeq, Jul 2008]Function:
catalytic activity:Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).cofactor:Magnesium.,disease:Very high levels of enzyme activity have been detected in certain acute leukemic cells.,function:Template-independent DNA polymerase which catalyzes the random addition of deoxynucleoside 5'-triphosphate to the 3'-end of a DNA initiator. One of the in vivo functions of this enzyme is the addition of nucleotides at the junction (N region) of rearranged Ig heavy chain and T-cell receptor gene segments during the maturation of B- and T-cells.,similarity:Belongs to the DNA polymerase type-X family.,similarity:Contains 1 BRCT domain.,subunit:Interacts with PRP19 and DNTTIP1. Forms a ternary complex with DNTTIP2 and core histone. Released from this complex by PCNASubcellular Location:
nuclear chromatin,euchromatin,nucleus,nucleoplasm,cytoplasm,nuclear matrix,
- Immunohistochemical analysis of paraffin-embedded Human Thyroid. 1, Antibody was diluted at 1:200(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min)
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