TCF-1 Polyclonal Antibody

Name: TCF-1 Polyclonal Antibody
Brand: EnoGene Biotech Co. Ltd
SKU: E20-74577
Price: 225 USD
Availability: InStock

E20-74577Cite ID: EN0054710Datasheet COA MSDS

SourcePolyclonal, Rabbit

$225/ 100ul

Quantity

Total: $225

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

OverviewImages & Data (2)Documents

Product Information

Molecular Weight(Da):41686

Protein NameTranscription factor 7

Human Gene Id6932

Human Swiss Prot NoP36402

Mouse Gene Id21414

Mouse Swiss Prot NoQ00417

SpecificityTCF-1 Polyclonal Antibody detects endogenous levels of TCF-1 protein.

FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration1 mg/ml

Storage Stability-20°C/1 year

Other NameTCF7; TCF1; Transcription factor 7; TCF-7; T-cell-specific transcription factor 1; T-cell factor 1; TCF-1

Observed Band(KD)42

Functionalternative products:2 series of isoforms, L and S, are produced by use of alternative promoter usage. Additional isoforms seem to exist,function:Transcriptional activator involved in T-cell lymphocyte differentiation. Necessary for the survival of CD4(+) CD8(+) immature thymocytes. Isoforms lacking the N-terminal CTNNB1 binding domain cannot fulfill this role. Binds to the T-lymphocyte-specific enhancer element (5'-WWCAAAG-3') found in the promoter of the CD3E gene. May also act as feedback transcriptional repressor of CTNNB1 and TCF7L2 target genes. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by TCF7 and CTNNB1.,induction:By TCF7L2 and CTNNB1.,sequence caution:Wrong choice of frame.,similarity:Belongs to the TCF/LEF family.,similarity:Contains 1 HMG box DNA-binding domain.,subunit:Binds the armadillo repeat of CTNNB1 and forms a stable complex. Interacts with AES, TLE1,

Subcellular LocationNucleus.

ExpressionPredominantly expressed in T-cells. Also detected in proliferating intestinal epithelial cells and in the basal epithelial cells of mammary gland epithelium.

PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

ImmunogenThe antiserum was produced against synthesized peptide derived from human TCF7. AA range:10-59

Gene NameTCF7

DilutionWestern Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.

Backgroundalternative products:2 series of isoforms, L and S, are produced by use of alternative promoter usage. Additional isoforms seem to exist,function:Transcriptional activator involved in T-cell lymphocyte differentiation. Necessary for the survival of CD4(+) CD8(+) immature thymocytes. Isoforms lacking the N-terminal CTNNB1 binding domain cannot fulfill this role. Binds to the T-lymphocyte-specific enhancer element (5'-WWCAAAG-3') found in the promoter of the CD3E gene. May also act as feedback transcriptional repressor of CTNNB1 and TCF7L2 target genes. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by TCF7 and CTNNB1.,induction:By TCF7L2 and CTNNB1.,sequence caution:Wrong choice of frame.,similarity:Belongs to the TCF/LEF family.,similarity:Contains 1 HMG box DNA-binding domain.,subunit:Binds the armadillo repeat of CTNNB1 and forms a stable complex. Interacts with AES, TLE1, TLE2, TLE3 and TLE4.,tissue specificity:Predominantly in T-cells. Also detected in proliferating intestinal epithelial cells and in the basal epithelial cells of mammary gland epithelium.,

📄Datasheet
Full product datasheet (PDF)✅Certificate of Analysis
Lot-specific COA (generated from product data)🧪MSDS
Material safety data sheet (generated)

Conjugated Antibody

This product is also available conjugated. Format: E20-74577-Conjugate. Price: $485 / Size: 100μl.

E20-74577-FITC$485 / 100μlE20-74577-Biotin$485 / 100μlE20-74577-HRP$485 / 100μlE20-74577-AP$485 / 100μlE20-74577-AF350$485 / 100μlE20-74577-AF405M$485 / 100μlE20-74577-AF488$485 / 100μlE20-74577-AF514$485 / 100μlE20-74577-AF532$485 / 100μlE20-74577-AF555$485 / 100μlE20-74577-AF568$485 / 100μlE20-74577-AF405S$485 / 100μlE20-74577-AF405L$485 / 100μlE20-74577-AF546$485 / 100μlE20-74577-AF594$485 / 100μlE20-74577-AF610$485 / 100μlE20-74577-AF635$485 / 100μlE20-74577-AF647$485 / 100μlE20-74577-AF680$485 / 100μlE20-74577-AF700$485 / 100μlE20-74577-AF750$485 / 100μlE20-74577-AF790$485 / 100μlE20-74577-APC$485 / 100μlE20-74577-PE$485 / 100μlE20-74577-Cy3$485 / 100μlE20-74577-Cy5$485 / 100μlE20-74577-Cy5.5$485 / 100μlE20-74577-Cy7$485 / 100μlE20-74577-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.