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Smad2 Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-74329

  • Amount:

    100ul

  • Price:

    225$

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human Smad2. AA range:418-467

  • Source:

    Polyclonal, Rabbit

  • Immunofluorescence analysis of Hela cell. 1,Smad2 Polyclonal Antibody(red) was diluted at 1:200(4° overnight). MICU1 Monoclonal Antibody(Mix)(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog:RS3611 was diluted at 1:1000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog:RS3208 was diluted at 1:1000(room temperature, 50min).

  • Gene Name:

    SMAD2

  • Dilution:

    Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.

  • Background:

    SMAD family member 2(SMAD2) Homo sapiens The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation

  • Protein Name:

    Mothers against decapentaplegic homolog 2

  • Human Gene Id:

    4087

  • Human Swiss Prot No:

    Q15796

  • Mouse Gene Id:

    17126

  • Mouse Swiss Prot No:

    Q62432

  • Rat Gene Id:

    29357

  • Rat Swiss Prot No:

    O70436

  • Specificity:

    Smad2 Polyclonal Antibody detects endogenous levels of Smad2 protein.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    SMAD2; MADH2; MADR2; Mothers against decapentaplegic homolog 2; MAD homolog 2; Mothers against DPP homolog 2; JV18-1; Mad-related protein 2; hMAD-2; SMAD family member 2; SMAD 2; Smad2; hSMAD2

  • MolecularWeight(Da):

    52306

  • Observed Band(KD):

    58

  • Function:

    disease:Defects in SMAD2 are found in sporadic cases of colorectal carcinoma.,function:Transcriptional modulator activated by TGF-beta and activin type 1 receptor kinase. SMAD2 is a receptor-regulated SMAD (R-SMAD). May act as a tumor suppressor in colorectal carcinoma.,PTM:Acetylated on Lys-19 by coactivators in response to TGF-beta signaling, which increases transcriptional activity. Isoform short: Acetylation increases DNA binding activity in vitro and enhances its association with target promoters in vivo.,PTM:In response to TGF-beta, ubiquitinated by NEDD4L; which promotes its degradation.,PTM:Phosphorylated on one or several of Thr-220, Ser-245, Ser-250, and Ser-255. In response to TGF-beta, phosphorylated on Ser-465/467 by TGF-beta and activin type 1 receptor kinases. Able to interact with SMURF2 when phosphorylated on Ser-465/467, recruiting other proteins, such as SNON, for degr

  • Subcellular Location:

    nuclear chromatin,nucleus,nucleoplasm,transcription factor complex,cytoplasm,cytosol,integral component of membrane,activin responsive factor complex,SMAD protein complex,SMAD2-SMAD3 protein complex,

  • Expression:

    Chronic myeloid leukemia cell,Colon adenocarcinoma,Epithelium,Kidney,Pancreas,Place

  • Western blot analysis of Smad2 Polyclonal Antibody, using Hela, HepG2,SW480, HEK293 cell, 4° over night, secondary antibody(cat: RS0002 was diluted at 1:10000, 37° 1hour.

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Catalog Number

  • Amount:100ul
  • Price:225$

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Smad2 Polyclonal Antibody | EnoGene Biotech Co. Ltd