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SLP-76 Polyclonal Antibody
Datasheet
Catalog Number:
E20-74321Amount:
100ulPurification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.Immunogen:
The antiserum was produced against synthesized peptide derived from human SLP-76. AA range:94-143Source:
Polyclonal, RabbitGene Name:
LCP2Dilution:
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.
- Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using SLP-76 Antibody. The picture on the right is blocked with the synthesized peptide.
Background:
lymphocyte cytosolic protein 2(LCP2) Homo sapiens SLP-76 was originally identified as a substrate of the ZAP-70 protein tyrosine kinase following T cell receptor (TCR) ligation in the leukemic T cell line Jurkat. The SLP-76 locus has been localized to human chromosome 5q33 and the gene structure has been partially characterized in mice. The human and murine cDNAs both encode 533 amino acid proteins that are 72% identical and comprised of three modular domains. The NH2-terminus contains an acidic region that includes a PEST domain and several tyrosine residues which are phosphorylated following TCR ligation. SLP-76 also contains a central proline-rich domain and a COOH-terminal SH2 domain. A number of additional proteins have been identified that associate with SLP-76 both constitutively and inducibly following receptor ligation, supporting the notion that SLP-76 functions as an adaptor or scaffold protein. Studies using SLP-76 deficient T cProtein Name:
Lymphocyte cytosolic protein 2Human Gene Id:
3937Human Swiss Prot No:
Q13094Mouse Gene Id:
16822Mouse Swiss Prot No:
Q60787Specificity:
SLP-76 Polyclonal Antibody detects endogenous levels of SLP-76 proteinFormulation:
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.Concentration:
1 mg/mlStorage Stability:
-20°C/1 yearOther Name:
LCP2; Lymphocyte cytosolic protein 2; SH2 domain-containing leukocyte protein of 76 kDa; SLP-76 tyrosine phosphoprotein; SLP76MolecularWeight(Da):
60188Observed Band(KD):
75Function:
domain:The SH2 domain mediates interaction with SHB.,function:Involved in T-cell antigen receptor mediated signaling.,PTM:Phosphorylated after T-cell receptor activation by ZAP-70.,similarity:Contains 1 SAM (sterile alpha motif) domain.,similarity:Contains 1 SH2 domain.,subunit:Interacts with SLA. Interacts with CBLB (By similarity). Interacts with the adapter proteins GRB2 and FYB. Interacts with SHB. Interacts with PRAM1.,tissue specificity:Highly expressed in spleen, thymus, and peripheral blood leukocytes. Highly expressed also in T-cell and monocytic cell lines, expressed at lower level in B-cell lines. Not detected in fibroblast or neuroblasatoma cell lines.,Subcellular Location:
cytosol,cell-cell junction,TCR signalosome,plasma membrane raft,Expression:
Leukemia,Platelet,Prostate,T-cell,
- Western blot analysis of lysates from Jurkat cells, treated with EGF 200ng/ml 5', using SLP-76 Antibody. The lane on the right is blocked with the synthesized peptide.
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