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POLR3H Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-73818

  • Amount:

    100ul

  • Reactivity:

    Human,Mouse,Rat

  • Purification:

    . The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human RPC8. AA range:151-200

  • Source:

    Polyclonal, Rabbit

  • Western Blot analysis of K562 cells using POLR3H Polyclonal Antibody diluted at 1:1000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).

  • Gene Name:

    POLR3H

  • Dilution:

    Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.

  • Protein Name:

    DNA-directed RNA polymerase III subunit RPC8

  • Human Gene Id:

    171568

  • Human Swiss Prot No:

    Q9Y535

  • Specificity:

    POLR3H Polyclonal Antibody detects endogenous levels of POLR3H protein.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    POLR3H; KIAA1665; RPC8; DNA-directed RNA polymerase III subunit RPC8; RNA polymerase III subunit C8; DNA-directed RNA polymerase III subunit H; RNA polymerase III subunit 22.9 kDa subunit; RPC22.9MolecularWeight: 22918Da

  • Observed Band(KD):

    23

  • Background:

    function:DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs.,similarity:Belongs to the eukaryotic RPB7/RPC8 RNA polymerase subunit family.,subunit:Component of the RNA polymerase III (Pol III) complex consisting of 17 subunits (By similarity). Interacts with CRCP/RPC9. POLR3H/RPC8 and CRCP/RPC9 probably form a Pol III subcomplex.,

  • Function:

    function:DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs.,similarity:Belongs to the eukaryotic RPB7/RPC8 RNA polymerase subunit family.,subunit:Component of the RNA polymerase III (Pol III) complex consisting of 17 subunits (By similarity). Interacts with CRCP/RPC9. POLR3H/RPC8 and CRCP/RPC9 probably form a Pol III subcomplex.,

  • Subcellular Location:

    nucleoplasm,DNA-directed RNA polymerase III complex,centrosome,cytosol,intracellular membrane-bounded organelle,

  • Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

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Catalog Number

  • Amount:100ul
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POLR3H Polyclonal Antibody | EnoGene Biotech Co. Ltd