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PHAX Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-73696

  • Amount:

    100ul

  • Price:

    225$

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human RNUXA. AA range:141-190

  • Source:

    Polyclonal, Rabbit

  • Gene Name:

    PHAX

  • Dilution:

    Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.

  • Western blot analysis of lysates from HT-29 cells, using RNUXA Antibody. The lane on the right is blocked with the synthesized peptide.

  • Background:

    function:A phosphoprotein adapter involved in the XPO1-mediated U snRNA export from the nucleus. Bridge components required for U snRNA export, the cap binding complex (CBC)-bound snRNA on the one hand and the GTPase Ran in its active GTP-bound form together with the export receptor XPO1 on the other. Its phosphorylation in the nucleus is required for U snRNA export complex assembly and export, while its dephosphorylation in the cytoplasm causes export complex disassembly. It is recycled back to the nucleus via the importin alpha/beta heterodimeric import receptor. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Its compartmentalized phosphorylation cycle may also contribute to the directionality of export. Binds strongly to m7G-capped U1 and U5 small nuclear RNAs (snRNAs) in a sequence-unspecific manner and phosphorylation-independent manner (By similarity). Plays also a role in the biogenesis of U3 small nucleolar RNA (snoRNA). Involved in the U3 snoRNA transport from nucleoplasm to Cajal bodies. Binds strongly to m7G-capped U3, U8 and U13 precursor snoRNAs and weakly to trimethylated (TMG)-capped U3, U8 and U13 snoRNAs. Binds also to telomerase RNA.,PTM:Phosphorylated in the nucleus. Dephosphorylated in the cytoplasm (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the PHAX family.,subcellular location:Located in the nucleoplasm and Cajal bodies. Shuttles between the nucleus and the cytoplasm. Shuttles between the nucleoplasm and Cajal bodies.,subunit:Found in a U snRNA export complex with PHAX/RNUXA, NCBP1, NCBP2, RAN, XPO1 and m7G-capped RNA. Part of a precomplex with PHAX/RNUXA, NCBP1, NCBP2 and m7G-capped RNA. Interacts with NCBP1 (By similarity). Found in a complex with snoRNA.,

  • Protein Name:

    Phosphorylated adapter RNA export protein

  • Human Gene Id:

    51808

  • Human Swiss Prot No:

    Q9H814

  • Mouse Swiss Prot No:

    Q9JJT9

  • Specificity:

    PHAX Polyclonal Antibody detects endogenous levels of PHAX protein.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    PHAX; RNUXA; Phosphorylated adapter RNA export protein; RNA U small nuclear RNA export adapter protein

  • MolecularWeight(Da):

    44403

  • Observed Band(KD):

    48

  • Function:

    function:A phosphoprotein adapter involved in the XPO1-mediated U snRNA export from the nucleus. Bridge components required for U snRNA export, the cap binding complex (CBC)-bound snRNA on the one hand and the GTPase Ran in its active GTP-bound form together with the export receptor XPO1 on the other. Its phosphorylation in the nucleus is required for U snRNA export complex assembly and export, while its dephosphorylation in the cytoplasm causes export complex disassembly. It is recycled back to the nucleus via the importin alpha/beta heterodimeric import receptor. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Its compartmentalized phosphorylation cycle may also contribute to the directionality of export. Binds strongly to m7G-capped U1 and U5 small nuclear RNAs (snRN

  • Subcellular Location:

    nucleus,nucleoplasm,centrosome,cytosol,Cajal body,neuronal cell body,intracellular membrane-bounded organelle,

  • Expression:

    Retinoblastoma,Skin,

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Catalog Number

  • Amount:100ul
  • Price:225$

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PHAX Polyclonal Antibody | EnoGene Biotech Co. Ltd