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Cell Signal Transduction Research Antibodies
p53RFP Polyclonal Antibody
Polyclonal, Rabbit
$225/ 100ul
QuantityTotal: $225
Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.
Product Information
Molecular Weight33697Da
Protein NameE3 ubiquitin-protein ligase RNF144B
Human Gene Id255488
Human Swiss Prot NoQ7Z419
Mouse Gene Id218215
Mouse Swiss Prot NoQ8BKD6
Specificityp53RFP Polyclonal Antibody detects endogenous levels of p53RFP protein.
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration1 mg/ml
Storage Stability-20°C/1 year
Other NameRNF144B; IBRDC2; P53RFP; E3 ubiquitin-protein ligase RNF144B; IBR domain-containing protein 2; RING finger protein 144B; p53-inducible RING finger protein
Observed Band(KD)35
Functioncaution:Lacks the His residue in the RING-type domain 2 that is one of the conserved features of the family.,domain:The RING-type zinc finger domain mediates binding to an E2 ubiquitin-conjugating enzyme.,function:E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates such as LCMT2, thereby promoting their degradation. Induces apoptosis via a TP53/p53-dependent but caspase-independent mechanism.,pathway:Protein modification; protein ubiquitination.,PTM:Auto-ubiquitinated.,similarity:Belongs to the RBR family. RNF144 subfamily.,similarity:Contains 1 IBR-type zinc finger.,similarity:Contains 2 RING-type zinc fingers.,subunit:Interacts with UBE2L3, UBE2L6 and LCMT2.,tissue specificity:Broadly expressed, with lowest levels in brain, spleen and thymu
Subcellular LocationMitochondrion membrane ; Single-pass membrane protein . Cytoplasm . Mostly cytosololic, accumulates in submitochondrial domains specifically upon apoptosis induction, in synchrony with BAX activation.
ExpressionBroadly expressed, with lowest levels in brain and thymus, and highest levels detectable in heart, ovary and testis.
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
ImmunogenSynthesized peptide derived from the Internal region of human p53RFP.
Gene NameRNF144B
DilutionWestern Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Backgroundcaution:Lacks the His residue in the RING-type domain 2 that is one of the conserved features of the family.,domain:The RING-type zinc finger domain mediates binding to an E2 ubiquitin-conjugating enzyme.,function:E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates such as LCMT2, thereby promoting their degradation. Induces apoptosis via a TP53/p53-dependent but caspase-independent mechanism.,pathway:Protein modification; protein ubiquitination.,PTM:Auto-ubiquitinated.,similarity:Belongs to the RBR family. RNF144 subfamily.,similarity:Contains 1 IBR-type zinc finger.,similarity:Contains 2 RING-type zinc fingers.,subunit:Interacts with UBE2L3, UBE2L6 and LCMT2.,tissue specificity:Broadly expressed, with lowest levels in brain, spleen and thymus.,
Conjugated Antibody
This product is also available conjugated. Format: E20-73544-Conjugate. Price: $485 / Size: 100μl.
E20-73544-FITC$485 / 100μlE20-73544-Biotin$485 / 100μlE20-73544-HRP$485 / 100μlE20-73544-AP$485 / 100μlE20-73544-AF350$485 / 100μlE20-73544-AF405M$485 / 100μlE20-73544-AF488$485 / 100μlE20-73544-AF514$485 / 100μlE20-73544-AF532$485 / 100μlE20-73544-AF555$485 / 100μlE20-73544-AF568$485 / 100μlE20-73544-AF405S$485 / 100μlE20-73544-AF405L$485 / 100μlE20-73544-AF546$485 / 100μlE20-73544-AF594$485 / 100μlE20-73544-AF610$485 / 100μlE20-73544-AF635$485 / 100μlE20-73544-AF647$485 / 100μlE20-73544-AF680$485 / 100μlE20-73544-AF700$485 / 100μlE20-73544-AF750$485 / 100μlE20-73544-AF790$485 / 100μlE20-73544-APC$485 / 100μlE20-73544-PE$485 / 100μlE20-73544-Cy3$485 / 100μlE20-73544-Cy5$485 / 100μlE20-73544-Cy5.5$485 / 100μlE20-73544-Cy7$485 / 100μlE20-73544-PE-Cy5$485 / 100μl
Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.
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