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Cell Signal Transduction Research Antibodies
p300 Polyclonal Antibody
Polyclonal, Rabbit
$225/ 100ul
QuantityTotal: $225
Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.
Product Information
Molecular Weight264144Da
Protein NameHistone acetyltransferase p300
Human Gene Id2033
Human Swiss Prot NoQ09472
Mouse Swiss Prot NoB2RWS6
Specificityp300 Polyclonal Antibody detects endogenous levels of p300 protein.
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration1 mg/ml
Storage Stability-20°C/1 year
Other NameEP300; P300; Histone acetyltransferase p300; p300 HAT; E1A-associated protein p300
Observed Band(KD)260
Functioncatalytic activity:Acetyl-CoA + histone = CoA + acetylhistone.,disease:Chromosomal aberrations involving EP300 may be a cause of acute myeloid leukemias. Translocation t(8;22)(p11;q13) with MYST3.,disease:Defects in EP300 are a cause of Rubinstein-Taybi syndrome (RSTS) [MIM:180849]. RSTS is an autosomal dominant disorder characterized by craniofacial abnormalities, broad thumbs, broad big toes, mental retardation and a propensity for development of malignancies.,disease:Defects in EP300 may play a role in epithelial cancer.,function:Functions as histone acetyltransferase and regulates transcription via chromatin remodeling. Acetylates all four core histones in nucleosomes. Histone acetylation gives an epigenetic tag for transcriptional activation. Binds to and may be involved in the transforming capacity of the adenovirus E1A protein. Mediates cAMP-gene regulation by binding specifically
Subcellular LocationCytoplasm . Nucleus . Chromosome . Localizes to active chromatin: Colocalizes with histone H3 acetylated and/or crotonylated at 'Lys-18' (H3K18ac and H3K18cr, respectively) (PubMed:25818647). In the presence of ALX1 relocalizes from the cytoplasm to the nucleus. Colocalizes with ROCK2 in the nucleus. .
ExpressionEpithelium,Skin,
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
ImmunogenThe antiserum was produced against synthesized peptide derived from human p300. AA range:55-104
Gene NameEP300
DilutionWestern Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.
BackgroundE1A binding protein p300(EP300) Homo sapiens This gene encodes the adenovirus E1A-associated cellular p300 transcriptional co-activator protein. It functions as histone acetyltransferase that regulates transcription via chromatin remodeling and is important in the processes of cell proliferation and differentiation. It mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein. This gene has also been identified as a co-activator of HIF1A (hypoxia-inducible factor 1 alpha), and thus plays a role in the stimulation of hypoxia-induced genes such as VEGF. Defects in this gene are a cause of Rubinstein-Taybi syndrome and may also play a role in epithelial cancer. [provided by RefSeq, Jul 2008],
Conjugated Antibody
This product is also available conjugated. Format: E20-73507-Conjugate. Price: $485 / Size: 100μl.
E20-73507-FITC$485 / 100μlE20-73507-Biotin$485 / 100μlE20-73507-HRP$485 / 100μlE20-73507-AP$485 / 100μlE20-73507-AF350$485 / 100μlE20-73507-AF405M$485 / 100μlE20-73507-AF488$485 / 100μlE20-73507-AF514$485 / 100μlE20-73507-AF532$485 / 100μlE20-73507-AF555$485 / 100μlE20-73507-AF568$485 / 100μlE20-73507-AF405S$485 / 100μlE20-73507-AF405L$485 / 100μlE20-73507-AF546$485 / 100μlE20-73507-AF594$485 / 100μlE20-73507-AF610$485 / 100μlE20-73507-AF635$485 / 100μlE20-73507-AF647$485 / 100μlE20-73507-AF680$485 / 100μlE20-73507-AF700$485 / 100μlE20-73507-AF750$485 / 100μlE20-73507-AF790$485 / 100μlE20-73507-APC$485 / 100μlE20-73507-PE$485 / 100μlE20-73507-Cy3$485 / 100μlE20-73507-Cy5$485 / 100μlE20-73507-Cy5.5$485 / 100μlE20-73507-Cy7$485 / 100μlE20-73507-PE-Cy5$485 / 100μl
Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.
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