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Cell Signal Transduction Research Antibodies
p27 Polyclonal Antibody
Polyclonal, Rabbit
$225/ 100ul
QuantityTotal: $225
Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.
Product Information
Molecular Weight22073Da
Protein NameCyclin-dependent kinase inhibitor 1B
Human Gene Id1027
Human Swiss Prot NoP46527
Mouse Gene Id12576
Mouse Swiss Prot NoP46414
Specificityp27 Polyclonal Antibody detects endogenous levels of p27 protein.
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration1 mg/ml
Storage Stability-20°C/1 year
Other NameCDKN1B; KIP1; Cyclin-dependent kinase inhibitor 1B; Cyclin-dependent kinase inhibitor p27; p27Kip1
Functiondisease:Defects in CDKN1B are the cause of multiple endocrine neoplasia type 4 (MEN4) [MIM:610755]. Multiple endocrine neoplasia (MEN) syndromes are inherited cancer syndromes of the thyroid. MEN4 is a MEN-like syndrome with a phenotypic overlap of both MEN1 and MEN2.,domain:A peptide sequence containing only AA 28-79 retains substantial Kip1 cyclin A/CDK2 inhibitory activity.,function:Important regulator of cell cycle progrssion. Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Positive regulator of cyclin D-dependent kinases such as CDK4. Regulated by phosphorylation and degradation events.,induction:Maximal levels in quiescence cells and early G(1). Levels decrease after mitogen stimulation as cells progress toward S-phase.,miscellaneous:Decreased levels of p27Kip1, mainly due to proteosomal degradation, are found in various epithelial tumors originati
Subcellular LocationNucleus. Cytoplasm. Endosome . Nuclear and cytoplasmic in quiescent cells. AKT- or RSK-mediated phosphorylation on Thr-198, binds 14-3-3, translocates to the cytoplasm and promotes cell cycle progression. Mitogen-activated UHMK1 phosphorylation on Ser-10 also results in translocation to the cytoplasm and cell cycle progression. Phosphorylation on Ser-10 facilitates nuclear export. Translocates to the nucleus on phosphorylation of Tyr-88 and Tyr-89. Colocalizes at the endosome with SNX6; this leads to lysosomal degradation (By similarity). .
ExpressionExpressed in kidney (at protein level) (PubMed:15509543). Expressed in all tissues tested. Highest levels in skeletal muscle, lowest in liver and kidney.
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
ImmunogenThe antiserum was produced against synthesized peptide derived from human p27 Kip1. AA range:1-50
Gene NameCDKN1B
DilutionWestern Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.
Backgroundcyclin dependent kinase inhibitor 1B(CDKN1B) Homo sapiens This gene encodes a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. Mutations in this gene are associated with multiple endocrine neoplasia type IV (MEN4). [provided by RefSeq, Apr 2014],
Conjugated Antibody
This product is also available conjugated. Format: E20-73502-Conjugate. Price: $485 / Size: 100μl.
E20-73502-FITC$485 / 100μlE20-73502-Biotin$485 / 100μlE20-73502-HRP$485 / 100μlE20-73502-AP$485 / 100μlE20-73502-AF350$485 / 100μlE20-73502-AF405M$485 / 100μlE20-73502-AF488$485 / 100μlE20-73502-AF514$485 / 100μlE20-73502-AF532$485 / 100μlE20-73502-AF555$485 / 100μlE20-73502-AF568$485 / 100μlE20-73502-AF405S$485 / 100μlE20-73502-AF405L$485 / 100μlE20-73502-AF546$485 / 100μlE20-73502-AF594$485 / 100μlE20-73502-AF610$485 / 100μlE20-73502-AF635$485 / 100μlE20-73502-AF647$485 / 100μlE20-73502-AF680$485 / 100μlE20-73502-AF700$485 / 100μlE20-73502-AF750$485 / 100μlE20-73502-AF790$485 / 100μlE20-73502-APC$485 / 100μlE20-73502-PE$485 / 100μlE20-73502-Cy3$485 / 100μlE20-73502-Cy5$485 / 100μlE20-73502-Cy5.5$485 / 100μlE20-73502-Cy7$485 / 100μlE20-73502-PE-Cy5$485 / 100μl
Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.
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