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Nucleophosmin Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-73208

  • Amount:

    100ul

  • Molecular Weight:

    32575Da

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human NPM. AA range:1-50

  • Source:

    Polyclonal, Rabbit

  • Western Blot analysis of COS7 cells using Nucleophosmin Polyclonal Antibody diluted at 1:2000

  • Gene Name:

    NPM1

  • Dilution:

    Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.

  • Protein Name:

    Nucleophosmin

  • Human Gene Id:

    4869

  • Human Swiss Prot No:

    P06748

  • Mouse Gene Id:

    18148

  • Mouse Swiss Prot No:

    Q61937

  • Rat Gene Id:

    25498

  • Rat Swiss Prot No:

    P13084

  • Specificity:

    Nucleophosmin Polyclonal Antibody detects endogenous levels of Nucleophosmin protein.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    NPM1; NPM; Nucleophosmin; NPM; Nucleolar phosphoprotein B23; Nucleolar protein NO38; Numatrin

  • Observed Band(KD):

    37

  • Background:

    nucleophosmin(NPM1) Homo sapiens This gene encodes a phosphoprotein which moves between the nucleus and the cytoplasm. The gene product is thought to be involved in several processes including regulation of the ARF/p53 pathway. A number of genes are fusion partners have been characterized, in particular the anaplastic lymphoma kinase gene on chromosome 2. Mutations in this gene are associated with acute myeloid leukemia. More than a dozen pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Nov 2009],

  • Function:

    disease:A chromosomal aberration involving NPM1 is a cause of myelodysplastic syndrome (MDS). Translocation t(3;5)(q25.1;q34) with MLF1.,disease:A chromosomal aberration involving NPM1 is found in a form of acute promyelocytic leukemia. Translocation t(5;17)(q32;q11) with RARA.,disease:A chromosomal aberration involving NPM1 is found in a form of non-Hodgkin lymphoma. Translocation t(2;5)(p23;q35) with ALK. The resulting chimeric NPM1-ALK protein homodimerize and the kinase becomes constitutively activated.,disease:Defects in NPM1 are associated with acute myelogenous leukemia (AML). Mutations in exon 12 affecting the C-terminus of the protein are associated with an aberrant cytoplasmic location.,function:Involved in diverse cellular processes such as ribosome biogenesis, centrosome duplication, protein chaperoning, histone assembly, cell proliferation, and regulation of tumor suppressor

  • Subcellular Location:

    Nucleus, nucleolus . Nucleus, nucleoplasm . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Generally nucleolar, but is translocated to the nucleoplasm in case of serum starvation or treatment with anticancer drugs. Has been found in the cytoplasm in patients with primary acute myelogenous leukemia (AML), but not with secondary AML. Can shuttle between cytoplasm and nucleus. Co- localizes with the methylated form of RPS10 in the granular component (GC) region of the nucleolus. Colocalized with nucleolin and APEX1 in nucleoli. Isoform 1 of NEK2 is required for its localization to the centrosome during mitosis.

  • Expression:

    Amnion,B-cell lymphoma,Bone marrow,Brain,Cervix carcinoma,Colon carcinoma,Epithelium,Kidney

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Catalog Number

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Nucleophosmin Polyclonal Antibody | EnoGene Biotech Co. Ltd