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Nicalin Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-73124

  • Amount:

    100ul

  • Swiss-Prot No.:

    Q969V3

  • Molecular Weight:

    62.9kd Observed Band 63kd

  • Applications:

    WB,IHC-p,IF(paraffin section),ELISA

  • Reactivity:

    Human,Mouse,Rat

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human NCLN. AA range:474-523

  • Specificity/Sensitivity:

    Nicalin Polyclonal Antibody detects endogenous levels of Nicalin protein.

  • Storage/Stability:

    -20°C/1 year

  • Source:

    Rabbit

  • Gene Name:

    NCLN

  • Dilution:

    Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.

  • Immunohistochemical analysis of paraffin-embedded Human colon cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

  • Gene ID:

    56926

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide

  • Function:

    May antagonize Nodal signaling and subsequent organization of axial structures during mesodermal patterning.,similarity:Belongs to the nicastrin family.,subunit:Interacts with NOMO2. Due to the strong similarity between NOMO1, NOMO2 and NOMO3, it probably also interacts with NOMO1 and NOMO3. Participates in a large protein complex, which is not related to the gamma-secretase complex.,tissue specificity:Highly expressed in pancreas and skeletal muscle and, at lower levels, in heart.,

  • Western blot analysis of lysates from HepG2 and HUVEC cells, using NCLN Antibody. The lane on the right is blocked with the synthesized peptide.

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Catalog Number

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