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NF-YB Polyclonal Antibody
Datasheet
Catalog Number:
E20-73091Amount:
100ulMolecular Weight:
22831DaPurification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.Immunogen:
The antiserum was produced against synthesized peptide derived from human NFYB. AA range:1-50Source:
Polyclonal, Rabbit
- Western blot analysis of lysates from 293 cells, using NFYB Antibody. The lane on the right is blocked with the synthesized peptide.
Gene Name:
NFYBDilution:
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.Protein Name:
Nuclear transcription factor Y subunit betaHuman Gene Id:
4801Human Swiss Prot No:
P25208Mouse Gene Id:
18045Mouse Swiss Prot No:
P63139Rat Gene Id:
25336Rat Swiss Prot No:
P63140Specificity:
NF-YB Polyclonal Antibody detects endogenous levels of NF-YB protein.Formulation:
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.Concentration:
1 mg/mlStorage Stability:
-20°C/1 yearOther Name:
NFYB; HAP3; Nuclear transcription factor Y subunit beta; CAAT box DNA-binding protein subunit B; Nuclear transcription factor Y subunit B; NF-YBObserved Band(KD):
29Background:
nuclear transcription factor Y subunit beta(NFYB) Homo sapiens The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoter regions in a variety of genes. This gene product, subunit B, forms a tight dimer with the C subunit, a prerequisite for subunit A association. The resulting trimer binds to DNA with high specificity and affinity. Subunits B and C each contain a histone-like motif. Observation of the histone nature of these subunits is supported by two types of evidence; protein sequence alignments and experiments with mutants. [provided by RefSeq, Jul 2008],Function:
domain:Can be divided into 3 domains: the weakly conserved A domain, the highly conserved B domain thought to be involved in subunit interaction and DNA binding, and the Glu-rich C domain.,function:Stimulates the transcription of various genes by recognizing and binding to a CCAAT motif in promoters, for example in type 1 collagen, albumin and beta-actin genes.,similarity:Belongs to the NFYB/HAP3 subunit family.,subunit:Heterotrimeric transcription factor composed of three components, NF-YA, NF-YB and NF-YC. NF-YB and NF-YC must interact and dimerize for NF-YA association and DNA binding.,Subcellular Location:
Nucleus.Expression:
Urinary bladder,
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