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NF-YA Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-73090

  • Amount:

    100ul

  • Molecular Weight:

    36877Da

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human NFYA. AA range:261-310

  • Source:

    Polyclonal, Rabbit

  • Western Blot analysis of various cells using NF-YA Polyclonal Antibody diluted at 1:1000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit

  • Gene Name:

    NFYA

  • Dilution:

    Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.

  • Protein Name:

    Nuclear transcription factor Y subunit alpha

  • Human Gene Id:

    4800

  • Human Swiss Prot No:

    P23511

  • Mouse Gene Id:

    18044

  • Mouse Swiss Prot No:

    P23708

  • Rat Gene Id:

    29508

  • Rat Swiss Prot No:

    P18576

  • Specificity:

    NF-YA Polyclonal Antibody detects endogenous levels of NF-YA protein.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    NFYA; Nuclear transcription factor Y subunit alpha; CAAT box DNA-binding protein subunit A; Nuclear transcription factor Y subunit A; NF-YA

  • Observed Band(KD):

    43

  • Background:

    nuclear transcription factor Y subunit alpha(NFYA) Homo sapiens The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds to CCAAT motifs in the promoter regions in a variety of genes. Subunit A associates with a tight dimer composed of the B and C subunits, resulting in a trimer that binds to DNA with high specificity and affinity. The sequence specific interactions of the complex are made by the A subunit, suggesting a role as the regulatory subunit. In addition, there is evidence of post-transcriptional regulation in this gene product, either by protein degradation or control of translation. Further regulation is represented by alternative splicing in the glutamine-rich activation domain, with clear tissue-specific preferences for the two isoforms. [provided by RefSeq, Jul 2008],

  • Function:

    function:Stimulates the transcription of various genes by recognizing and binding to a CCAAT motif in promoters, for example in type 1 collagen, albumin and beta-actin genes.,similarity:Belongs to the NFYA/HAP2 subunit family.,subunit:Heterotrimeric transcription factor composed of three components, NF-YA, NF-YB and NF-YC. NF-YB and NF-YC must interact and dimerize for NF-YA association and DNA binding.,

  • Subcellular Location:

    Nucleus.

  • Expression:

    Testis,

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Catalog Number

  • Amount:100ul
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