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NF-1 Polyclonal Antibody
Datasheet
Catalog Number:
E20-73078Amount:
100ulPrice:
225$Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.Immunogen:
The antiserum was produced against synthesized peptide derived from human Nuclear Factor 1. AA range:11-60Source:
Polyclonal, RabbitGene Name:
NFIADilution:
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.
- Western blot analysis of lysates from rat brain and HUVEC cells, using Nuclear Factor 1 Antibody. The lane on the right is blocked with the synthesized peptide.
Background:
nuclear factor I A(NFIA) Homo sapiens This gene encodes a member of the NF1 (nuclear factor 1) family of transcription factors. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011],Protein Name:
Nuclear factor 1 A-typeHuman Gene Id:
4774/4781/4782/4784Human Swiss Prot No:
Q12857/O00712/P08651/Q14938Mouse Gene Id:
18027/18028/18029/18032Rat Gene Id:
25492Rat Swiss Prot No:
P09414Specificity:
NF-1 Polyclonal Antibody detects endogenous levels of NF-1 protein.Formulation:
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.Concentration:
1 mg/mlStorage Stability:
-20°C/1 yearOther Name:
NFIA; KIAA1439; Nuclear factor 1 A-type; NF1-A; Nuclear factor 1/A; CCAAT-box-binding transcription factor; CTF; Nuclear factor I/A; NF-I/A; NFI-A; TGGCA-binding protein; NFIB; Nuclear factor 1 B-type; NF1-B; Nuclear factor 1/B; CCAAT-box-bMolecularWeight(Da):
55944Observed Band(KD):
55Function:
function:Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the CTF/NF-I family.,similarity:Contains 1 CTF/NF-I DNA-binding domain.,subunit:Binds DNA as a homodimer.,Subcellular Location:
nucleus,nucleoplasm,cell junction,Expression:
Brain,Epithelium,Skeletal muscle,Testis,
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Catalog Number
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