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Nek7 Polyclonal Antibody
Datasheet
Catalog Number:
E20-73034Amount:
100ulMolecular Weight:
34551DaPurification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.Immunogen:
The antiserum was produced against synthesized peptide derived from human NEK7. AA range:101-150Source:
Polyclonal, RabbitGene Name:
NEK7Dilution:
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.
- Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using NEK7 Antibody. The picture on the right is blocked with the synthesized peptide.
Protein Name:
Serine/threonine-protein kinase Nek7Human Gene Id:
140609Human Swiss Prot No:
Q8TDX7Mouse Gene Id:
59125Mouse Swiss Prot No:
Q9ES74Specificity:
Nek7 Polyclonal Antibody detects endogenous levels of Nek7 protein.Formulation:
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.Concentration:
1 mg/mlStorage Stability:
-20°C/1 yearOther Name:
NEK7; Serine/threonine-protein kinase Nek7; Never in mitosis A-related kinase 7; NimA-related protein kinase 7Observed Band(KD):
35Background:
NIMA related kinase 7(NEK7) Homo sapiens NIMA-related kinases share high amino acid sequence identity with the gene product of the Aspergillus nidulans 'never in mitosis A' gene, which controls initiation of mitosis.[supplied by OMIM, Jul 2002],Function:
catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.,similarity:Contains 1 protein kinase domain.,tissue specificity:Highly expressed in lung, muscle, testis, brain, heart, liver, leukocyte and spleen. Lower expression in ovary, prostate and kidney. No expression seen in small intestine.,Subcellular Location:
spindle pole,nucleoplasm,cytoplasm,microtubule organizing center,microtubule,Expression:
Uterus,
- Western blot analysis of lysates from HepG2, Jurkat, and COLO cells, using NEK7 Antibody. The lane on the right is blocked with the synthesized peptide.
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