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N-cadherin Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-72988

  • Amount:

    100ul

  • Molecular Weight:

    99809Da

  • Applications:

    WB;IHC-p;IF/ICC;ELISA

  • Reactivity:

    Human;Mouse;Rat

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human CDH2. AA range:721-770

  • Source:

    Polyclonal, Rabbit

  • Western Blot analysis of K562 cells using N-cadherin Polyclonal Antibody diluted at 1:1000

  • Gene Name:

    CDH2

  • Dilution:

    Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.

  • Protein Name:

    Cadherin-2

  • Human Gene Id:

    1000

  • Human Swiss Prot No:

    P19022

  • Mouse Gene Id:

    12558

  • Mouse Swiss Prot No:

    P15116

  • Rat Gene Id:

    83501

  • Rat Swiss Prot No:

    Q9Z1Y3

  • Specificity:

    N-cadherin Polyclonal Antibody detects endogenous levels of N-cadherin protein.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    CDH2; CDHN; NCAD; Cadherin-2; CDw325; Neural cadherin; N-cadherin; CD antigen CD325

  • Observed Band(KD):

    140

  • Background:

    cadherin 2(CDH2) Homo sapiens This gene encodes a classical cadherin and member of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein is proteolytically processed to generate a calcium-dependent cell adhesion molecule and glycoprotein. This protein plays a role in the establishment of left-right asymmetry, development of the nervous system and the formation of cartilage and bone. [provided by RefSeq, Nov 2015],

  • Function:

    function:Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH2 may be involved in neuronal recognition mechanism.,similarity:Contains 5 cadherin domains.,subunit:Interacts with CDCP1.,

  • Subcellular Location:

    Cell membrane ; Single-pass type I membrane protein . Cell membrane, sarcolemma . Cell junction . Cell surface . Colocalizes with TMEM65 at the intercalated disk in cardiomyocytes. Colocalizes with OBSCN at the intercalated disk and at sarcolemma in cardiomyocytes. .

  • Expression:

    Brain,Epithelium,Liver,

  • Immunohistochemical analysis of paraffin-embedded Human kidney. 1, Antibody was diluted at 1:200(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).

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Catalog Number

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N-cadherin Polyclonal Antibody | EnoGene Biotech Co. Ltd