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N/H/K-Ras Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-72960

  • Amount:

    100ul

  • Molecular Weight:

    21298/21656Da

  • Applications:

    WB;IHC-p;IF/ICC;ELISA

  • Reactivity:

    Human;Mouse;Rat

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human RASH/RASK. AA range:1-50

  • Source:

    Polyclonal, Rabbit

  • Western Blot analysis of 293 cells using N/H/K-Ras Polyclonal Antibody diluted at 1:1000

  • Gene Name:

    NRAS/HRAS/KRAS

  • Dilution:

    Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. IF 1:100-300 Not yet tested in other applications.

  • Protein Name:

    GTPase Nras/GTPase Hras/GTPase Kras

  • Human Gene Id:

    3265/3845/4893

  • Human Swiss Prot No:

    P01111/P01112/P01116

  • Mouse Gene Id:

    15461/16653

  • Rat Gene Id:

    24605/293621/24525

  • Rat Swiss Prot No:

    Q04970/P20171/P08644

  • Specificity:

    N/H/K-Ras Polyclonal Antibody detects endogenous levels of N/H/K-Ras protein.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    NRAS; HRAS1; GTPase NRas; Transforming protein N-Ras; HRAS; HRAS1; GTPase HRas; H-Ras-1; Ha-Ras; Transforming protein p21; c-H-ras; p21ras; KRAS; KRAS2; RASK2; GTPase KRas; K-Ras 2; Ki-Ras; c-K-ras; c-Ki-ras

  • Observed Band(KD):

    21

  • Background:

    neuroblastoma RAS viral oncogene homolog(NRAS) Homo sapiens This is an N-ras oncogene encoding a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. This shuttling is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex. The encoded protein, which has intrinsic GTPase activity, is activated by a guanine nucleotide-exchange factor and inactivated by a GTPase activating protein. Mutations in this gene have been associated with somatic rectal cancer, follicular thyroid cancer, autoimmune lymphoproliferative syndrome, Noonan syndrome, and juvenile myelomonocytic leukemia. [provided by RefSeq, Jun 2011],

  • Function:

    disease:Defects in NRAS are a cause of juvenile myelomonocytic leukemia (JMML) [MIM:607785]. JMML is a pediatric myelodysplastic syndrome that constitutes approximately 30% of childhood cases of myelodysplastic syndrome (MDS) and 2% of leukemia.,disease:Mutations which change AA 12, 13 or 61 activate the potential of Ras to transform cultured cells and are implicated in a variety of human tumors.,enzyme regulation:Alternate between an inactive form bound to GDP and an active form bound to GTP. Activated by a guanine nucleotide-exchange factor (GEF) and inactivated by a GTPase-activating protein (GAP).,function:Ras proteins bind GDP/GTP and possess intrinsic GTPase activity.,online information:NRAS mutation db,online information:RAS proteins entry,PTM:Palmitoylated by the ZDHHC9-GOLGA7 complex. A continuous cycle of de- and re-palmitoylation regulates rapid exchange between plasma membran

  • Subcellular Location:

    Cell membrane ; Lipid-anchor ; Cytoplasmic side . Golgi apparatus membrane ; Lipid-anchor . Shuttles between the plasma membrane and the Golgi apparatus. .

  • Expression:

    Bone marrow,Bone-marrow,Brain,Fibrosarcoma,Kidney,Leukemia,Lung car

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Catalog Number

  • Amount:100ul
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N/H/K-Ras Polyclonal Antibody | EnoGene Biotech Co. Ltd