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MMP-14 Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-72910

  • Amount:

    100ul

  • Price:

    225$

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human MMP-14. AA range:471-520

  • Source:

    Polyclonal, Rabbit

  • Gene Name:

    MMP14

  • Dilution:

    Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.

  • Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using MMP-14 Antibody. The picture on the right is blocked with the synthesized peptide.

  • Background:

    matrix metallopeptidase 14(MMP14) Homo sapiens Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the protein encoded by this gene is a member of the membrane-type MMP (MT-MMP) subfamily; each member of this subfamily contains a potential transmembrane domain suggesting that these proteins are expressed at the cell surface rather than secreted. This protein activates MMP2 protein, and this activity may be involved in tumor invasion. [provided by RefSeq, Jul 2008],

  • Protein Name:

    Matrix metalloproteinase-14

  • Human Gene Id:

    4323

  • Human Swiss Prot No:

    P50281

  • Mouse Gene Id:

    17387

  • Mouse Swiss Prot No:

    P53690

  • Rat Gene Id:

    81707

  • Rat Swiss Prot No:

    Q10739

  • Specificity:

    MMP-14 Polyclonal Antibody detects endogenous levels of MMP-14 protein.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    MMP14; Matrix metalloproteinase-14; MMP-14; MMP-X1; Membrane-type matrix metalloproteinase 1; MT-MMP 1; MTMMP1; Membrane-type-1 matrix metalloproteinase; MT1-MMP; MT1MMP

  • MolecularWeight(Da):

    65884

  • Observed Band(KD):

    65

  • Function:

    catalytic activity:Endopeptidase activity. Activates progelatinase A by cleavage of the propeptide at 37-Asn-|-Leu-38. Other bonds hydrolyzed include 35-Gly-|-Ile-36 in the propeptide of collagenase 3, and 341-Asn-|-Phe-342, 441-Asp-|-Leu-442 and 354-Gln-|-Thr-355 in the aggrecan interglobular domain.,cofactor:Binds 1 zinc ion per subunit.,cofactor:Calcium.,domain:The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.,function:Seems to specifically activate progelatinase A. May thus trigger invasion by tumor cells by activating progelatinase A on the tumor cell surface.,PTM:The precursor is cleaved by a furin endopeptidase.,similarity:Belongs to the peptidase M10A family.,similarity:Contains 4 hemopexin-like domains.,s

  • Subcellular Location:

    cytoplasm,Golgi lumen,plasma membrane,integral component of plasma membrane,focal adhesion,integral component of membrane,extracellular matrix,cytoplasmic vesicle,melanosome,macropinosome,

  • Expression:

    Lung,Placenta,Skin,Tongue,

  • Western blot analysis of lysates from NIH/3T3 cells, using MMP-14 Antibody. The lane on the right is blocked with the synthesized peptide.

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Catalog Number

  • Amount:100ul
  • Price:225$

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MMP-14 Polyclonal Antibody | EnoGene Biotech Co. Ltd