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MRP-L47 Polyclonal Antibody
Datasheet
Catalog Number:
E20-72866Amount:
100ulSwiss-Prot No.:
Q9HD33Molecular Weight:
29.5kd Observed Band 30kdApplications:
WB,ELISAReactivity:
HumanPurification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.Immunogen:
The antiserum was produced against synthesized peptide derived from human MRPL47. AA range:191-240Specificity/Sensitivity:
MRP-L47 Polyclonal Antibody detects endogenous levels of MRP-L47 protein.Storage/Stability:
-20°C/1 yearSource:
RabbitGene Name:
MRPL47Dilution:
Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
- Western blot analysis of lysates from RAW264.7 and K562 cells, using MRPL47 Antibody. The lane on the right is blocked with the synthesized peptide.
Background:
mitochondrial ribosomal protein L47(MRPL47) Homo sapiens Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. This gene is immediately adjacent to the gene for BAF complex 53 kDa subunit protein a (BAF53a), in a tail-to-tail orientation. Two transcript variants encoding different protein isoforms have been identifieGene ID:
57129Formulation:
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azideFunction:
sequence caution:Chimeric cDNA. Seems to include a portion of the orthologous murine sequence.,similarity:Belongs to the ribosomal protein L47em family.,
- Western blot analysis of the lysates from 293 cells using MRPL47 antibody.
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