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MRP-L4 Polyclonal Antibody
Datasheet
Catalog Number:
E20-72861Amount:
100ulMolecular Weight:
34919DaApplications:
WB;IHC-p;IF(paraffin section);ELISAReactivity:
Human;MousePurification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.Immunogen:
The antiserum was produced against synthesized peptide derived from human MRPL4. AA range:90-139Source:
Polyclonal, Rabbit,IgG
- Western Blot analysis of various cells using MRP-L4 Polyclonal Antibody
Gene Name:
MRPL4Dilution:
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.Protein Name:
39S ribosomal protein L4 mitochondrialHuman Gene Id:
51073Human Swiss Prot No:
Q9BYD3Mouse Gene Id:
66163Mouse Swiss Prot No:
Q9DCU6Specificity:
MRP-L4 Polyclonal Antibody detects endogenous levels of MRP-L4 protein.Formulation:
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.Concentration:
1 mg/mlStorage Stability:
-20°C/1 yearOther Name:
MRPL4; CDABP0091; CGI-28; 39S ribosomal protein L4; mitochondrial; L4mt; MRP-L4Observed Band(KD):
35Background:
mitochondrial ribosomal protein L4(MRPL4) Homo sapiens Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. Sequence analysis identified alternatively spliced variants that encode different protein isoforms. [provided by RefSeq, Jul 2008],Function:
similarity:Belongs to the ribosomal protein L4P family.,Subcellular Location:
Mitochondrion .Expression:
Leukemia,Lung,Ovary,
- Immunohistochemical analysis of paraffin-embedded Human lung cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
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